Plasmacytoid dendritic cells sense skin injury and promote wound healing through type I interferons
| Autor: | Anna Di Nardo, Josh Gregorio, Richard L. Gallo, John DiGiovanni, Antti Lauerma, Naoko Arai, Michel Gilliet, Curdin Conrad, Stephan Meller, Bernhard Homey |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Male
Mice 129 Strain Molecular Sequence Data Immunology Human skin Receptor Interferon alpha-beta Biology Article Mice 03 medical and health sciences 0302 clinical medicine Immune system Cathelicidins Interferon Nucleic Acids Sense (molecular biology) medicine Animals Humans Immunology and Allergy Amino Acid Sequence Skin 030304 developmental biology Mice Knockout Mice Inbred BALB C Wound Healing 0303 health sciences Membrane Glycoproteins integumentary system Reverse Transcriptase Polymerase Chain Reaction TLR9 virus diseases hemic and immune systems Dendritic Cells TLR7 3. Good health Mice Inbred C57BL Toll-Like Receptor 7 Interferon Type I Myeloid Differentiation Factor 88 Cytokines Female Wound healing Interferon type I Antimicrobial Cationic Peptides 030215 immunology medicine.drug |
| Zdroj: | The Journal of Experimental Medicine |
| ISSN: | 1540-9538 0022-1007 |
| Popis: | Cutaneous injury in mice drives transient TLR7- and TLR9-mediated production of type I interferon by plasmacytoid dendritic cells, which is required for re-epithelialization of the skin. Plasmacytoid dendritic cells (pDCs) are specialized type I interferon (IFN-α/β)–producing cells that express intracellular toll-like receptor (TLR) 7 and TLR9 and recognize viral nucleic acids in the context of infections. We show that pDCs also have the ability to sense host-derived nucleic acids released in common skin wounds. pDCs were found to rapidly infiltrate both murine and human skin wounds and to transiently produce type I IFNs via TLR7- and TLR9-dependent recognition of nucleic acids. This process was critical for the induction of early inflammatory responses and reepithelization of injured skin. Cathelicidin peptides, which facilitate immune recognition of released nucleic acids by promoting their access to intracellular TLR compartments, were rapidly induced in skin wounds and were sufficient but not necessary to stimulate pDC activation and type I IFN production. These data uncover a new role of pDCs in sensing tissue damage and promoting wound repair at skin surfaces. |
| Databáze: | OpenAIRE |
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