Pancreatic Islet Mitochondrial Glycerophosphate Dehydrogenase Deficiency in Two Animal Models of Non-Insulin-Dependent Diabetes Mellitus
| Autor: | Marie-Hélène Giroix, Anna Novials, Abdullah Sener, Marta E Fabregat, Willy Malaisse, Ramon Gomis |
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| Rok vydání: | 1996 |
| Předmět: |
Blood Glucose
endocrine system medicine.medical_specialty endocrine system diseases Period (gene) Blotting Western Biophysics Glycerolphosphate Dehydrogenase Biochemistry Rats Mutant Strains Diabetes Mellitus Experimental Islets of Langerhans Reference Values Internal medicine Glucokinase medicine Animals Insulin Endocrine system Rats Wistar Molecular Biology chemistry.chemical_classification geography geography.geographical_feature_category Cell Biology Streptozotocin Islet Mitochondria Rats Blot Enzyme medicine.anatomical_structure Endocrinology Diabetes Mellitus Type 2 chemistry Pancreas medicine.drug |
| Zdroj: | Biochemical and Biophysical Research Communications. 220:1020-1023 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1996.0525 |
| Popis: | Western blotting of pancreatic islet extracts from either hereditarily diabetic Goto-Kakizaki rats (GK rats) or animals injected with streptozotocin during the neonatal period (STZ rats) demonstrated a pronounced decrease of immunoreactive mitochondrial glycerophosphate dehydrogenase (m-GDH), when compared to results obtained in islets from control rats. By contrast, the islet glucokinase protein content was either unaffected (GK rats) or much less severely decreased than that of m-GDH (STZ rats). These findings indicate that the impaired activity of m-GDH previously documented in islet homogenates from diabetic rats coincides with a decreased content of this enzyme in the endocrine pancreas. |
| Databáze: | OpenAIRE |
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