Growth of 28 Legionella species on selective culture media: a comparative study

Autor:	Victor L. Yu, Marilyn M. Wagener, R M Vickers, T C Lee
Rok vydání:	1993
Předmět:	Microbiology (medical) food.ingredient biology Legionella biology.organism_classification Legionella pneumophila Culture Media respiratory tract diseases Microbiology food medicine Agar Vancomycin Cefamandole Legionella species Bacteria Polymyxin B Research Article medicine.drug
Zdroj:	Journal of Clinical Microbiology. 31:2764-2768
ISSN:	1098-660X 0095-1137
Popis:	We compared the growth of 28 Legionella spp. on four manufacturers' buffered charcoal-yeast extract (BCYE) agar media and selective BCYE media that contained polymyxin B, anisomycin, and vancomycin or cefamandole. With BCYE as a "gold standard," growth for Legionella pneumophila was significantly better than for the nonpneumophila species on all media tested. L. pneumophila and 24 other Legionella spp. grew on vancomycin-containing media, while L. santicrucis, L. rubrilucens, and L. erythra grew poorly. In contrast, 11 of 28 species (notably L. micdadei and L. bozemanii) did not grow on cefamandole-containing media and 8 of 28 species only grew marginally. We demonstrated that selective BCYE media that contain vancomycin or cefamandole may not support the growth of all Legionella spp. One commercial manufacturer's media were consistently suboptimal. Laboratories should not rely on a manufacturer's quality control testing in lieu of their own.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d0cb7e796a092e8460384efa5564ff75 https://doi.org/10.1128/jcm.31.10.2764-2768.1993 Zobrazit plný text záznamu