| Autor: |
Zhu, Bo, Hu, Juan, Li, Xuelian, Li, Xiaomin, Wang, Lei, Fan, Shihui, Jin, Xin, Wang, Kun, Zhao, Weiguo, Zhu, Wenjun, Chen, Cheng, Wang, Zilu, Lu, Yingzhi |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Frontiers in Cellular and Infection Microbiology. 12 |
| ISSN: |
2235-2988 |
| DOI: |
10.3389/fcimb.2022.991849 |
| Popis: |
Enterococcus faecalis is a serious problem for hospitals and can spread from patient to patient. Most of the current detection methods are associated with limitations associated with the need for trained personnel; they are also time-consuming. Thus, it is necessary to develop rapid and accurate detection methods to control the spread of E. faecalis. In this study, we developed a rapid and accurate detection method for E. faecalis using recombinase polymerase amplification (RPA) combined with a lateral flow strip (LFS). This method could be completed in approximately 35 min at 37°C. The limit of detection was 10 CFU/µL, irrespective of whether the templates were pure or complex. This method also showed good specificity and compatibility. In total, 278 clinical samples were tested using the RPA-LFS method; the detection accuracy was equal to that of the conventional qPCR method. This visualized isothermal amplification method could be useful for the future on-site detection of E. faecalis. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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