Pancreatic carcinoma cells are susceptible to noninvasive radio frequency fields after treatment with targeted gold nanoparticles
| Autor: | Steven A. Curley, Cihui Zhu, Evan S. Glazer, Katheryn L. Massey |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Pathology
medicine.medical_specialty Cell Survival Cell Cetuximab Metal Nanoparticles Antibodies Monoclonal Humanized Article Flow cytometry Microscopy Electron Transmission Epidermal growth factor Cell Line Tumor medicine Humans medicine.diagnostic_test business.industry Antibodies Monoclonal Cancer Hyperthermia Induced Radiofrequency Therapy medicine.disease ErbB Receptors Pancreatic Neoplasms medicine.anatomical_structure Cell culture Colloidal gold Cancer cell Cancer research Surgery Gold business medicine.drug |
| Zdroj: | Surgery. 148:319-324 |
| ISSN: | 0039-6060 |
| Popis: | Gold and carbon nanoparticles absorb nonionizing radio frequency (RF) energy and release heat. Solid gold nanoparticles are delivered to cancer cells via conjugation with targeting antibodies. Here, 20-nm gold particles were conjugated to cetuximab, which is an epidermal growth factor receptor-1 (EGFR-1) antibody.A pancreatic carcinoma cell line that highly expresses EGFR-1, Panc-1, and Cama-1, which is a breast carcinoma cell line that minimally expresses EGFR-1, were treated with 100-nmol/L cetuximab-conjugated gold nanoparticles for 3 h (n = 4). Thirty-six hours later, the dishes were placed in an RF field with a generator power of 200 W for 5 min. After another 36 h, cell injury and death were evaluated with flow cytometry.The targeted cell line Panc-1 had a viability of 46% +/- 12%, whereas the Cama-1 cell had a viability of 92% +/- 2% after RF field exposure (P.008). Transmission electron microscopy showed gold nanoparticle uptake in Panc-1 cells but negligible uptake by Cama-1 cells. Nontargeted cells do not internalize a sufficient amount of antibody-conjugated gold nanoparticles to induce injury in a noninvasive RF field.This technique could be useful in cancer treatment if a cancer-specific antibody is used to localize gold nanoparticles to malignant cells. |
| Databáze: | OpenAIRE |
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