Canine coronavirus infection in the dog following oronasal inoculation
| Autor: | B. J. Tennant, C. J. Gaskell, D. F. Kelly, Stuart D. Carter, Rosalind M. Gaskell |
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| Rok vydání: | 1991 |
| Předmět: |
Diarrhea
Colon Coronaviridae Coronaviridae Infections Duodenum Palatine Tonsil Immunoglobulins Oropharynx Antibodies Viral medicine.disease_cause Article Virus Campylobacter jejuni Feces Dogs Campylobacter Infections Intestine Small medicine Animals Dog Diseases Viral shedding Coronavirus General Veterinary biology Inoculation Rectum Canine coronavirus biology.organism_classification Immunohistochemistry Virology Lymphatic system biology.protein Antibody |
| Zdroj: | Research in Veterinary Science |
| ISSN: | 0034-5288 |
| DOI: | 10.1016/0034-5288(91)90023-h |
| Popis: | The pathogenesis of canine coronavirus (CCV) infection in 10-week-old puppies was studied up to 14 days after oronasal inoculation. Mild diarrhoea was seen from three to 11 days after inoculation, approximately coincident with faecal virus shedding. Virus was initially isolated from the tonsils on day 3, and then from both small and large intestinal tissues up to 14 days after inoculation. Virus was also isolated from liver and lung. Histological changes were not seen in any tissues, but CCV antigen was detected, using a peroxidase antiperoxidase staining technique, mainly in epithelium overlying gut-associated lymphoid tissue. Virus neutralising antibody was first detected on day 10. Specific anti-CCV IgM was first detected in plasma three days after inoculation and IgG on days 4 to 7. Small amounts of anti-CCV IgG, IgM and IgA were detected in duodenal secretion, but none in bile. |
| Databáze: | OpenAIRE |
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