Construction and analysis of self-cloning sake yeasts that accumulate proline
Autor: | Akari Kawaguchi, Yoshito Kubo, Hiroshi Takagi, Hitoshi Shimoi, Hong Wu, Fumi Matsui |
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Rok vydání: | 2007 |
Předmět: |
Cloning
Ethanol Proline biology Strain (chemistry) Alcoholic Beverages Saccharomyces cerevisiae food and beverages Bioengineering biology.organism_classification Applied Microbiology and Biotechnology Yeast chemistry.chemical_compound Species Specificity chemistry Biochemistry Food Microbiology Fermentation Ethanol fuel Biotechnology |
Zdroj: | Journal of Bioscience and Bioengineering. 103:377-380 |
ISSN: | 1389-1723 |
DOI: | 10.1263/jbb.103.377 |
Popis: | We constructed self-cloning diploid sake yeast strains that accumulate proline. The appropriate proline level is important for its protective effect against ethanol stress in yeast cells. Sake brewed with the proline-accumulating strains contained two- to threefold more proline than the sake brewed with the parent strain. It was also suggested that intracellular proline does not affect overall fermentation profiles, but reduces fermentation time in terms of ethanol production rate. |
Databáze: | OpenAIRE |
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