Rat Kidney Lysosomal Membrane Damage Induced by Suramin in Vitro and in Vivo
| Autor: | Akanji Ma |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Male
Light Health Toxicology and Mutagenesis Suramin Acid Phosphatase Kidney Toxicology chemistry.chemical_compound Naphthylamine In vivo Lysosome polycyclic compounds medicine Animals Pharmacology biology Acid phosphatase Intracellular Membranes In vitro Rats medicine.anatomical_structure chemistry Biochemistry biology.protein Muramidase Lysozyme Lysosomes medicine.drug |
| Zdroj: | Pharmacology & Toxicology. 62:318-321 |
| ISSN: | 1600-0773 0901-9928 |
| DOI: | 10.1111/j.1600-0773.1988.tb01895.x |
| Popis: | The effect of suramin, an acid naphthylamine, on rat kidney lysosomal membrane integrity was studied. Lysosomal particles were incubated with suramin (0.1-0.4 mM) and light scattering behaviour of the mixtures were subsequently measured. There was a significant decrease (P less than 0.005) in the amount of light absorbed in the presence of suramin compared to lysosomal particle suspension alone. This was accompanied by release of acid phosphatase, a lysosomal 'marker' enzyme, into the suspending medium. These effects were reduced in the presence of acetylsalicylic acid, a known lysosomal membrane stabilizer. Administration of suramin to rats resulted in loss of kidney acid phosphatase and lysozyme activities from the tissue. These results indicate labilization of rat kidney lysosomal membrane by suramin molecules both in vitro and in vivo. |
| Databáze: | OpenAIRE |
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