Red Light-Induced Phytochrome Relocation into the Nucleus in Adiantum capillus-veneris
| Autor: | Eberhard Schäfer, Sachihiko Nakamura, Masamitsu Wada, Hidenori Tsuboi |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Cell Nucleus
Light Transcription Genetic biology Phytochrome Recombinant Fusion Proteins Adiantum fungi Intracellular Space Germination Far-red Plant Science biology.organism_classification Adiantum capillus-veneris Spore Protein Transport Gene Expression Regulation Plant Darkness Botany Spore germination Molecular Biology |
| Zdroj: | Molecular Plant. 5:611-618 |
| ISSN: | 1674-2052 |
| DOI: | 10.1093/mp/ssr119 |
| Popis: | Phytochromes in seed plants are known to move into nuclei in a red light-dependent manner with or without interacting factors. Here, we show phytochrome relocation to the nuclear region in phytochrome-dependent Adiantum capillus-veneris spore germination by partial spore-irradiation experiments. The nuclear or non-nuclear region of imbibed spores was irradiated with a microbeam of red and/or far-red light and the localization of phytochrome involved in spore germination was estimated from the germination rate. The phytochrome for spore germination existed throughout whole spore under darkness after imbibition, but gradually migrated to the nuclear region following red light irradiation. Intracellular distribution of PHY–GUS fusion proteins expressed in germinated spores by particle bombardment showed the migration of Ac phy2, but not Ac phy1, into nucleus in a red light-dependent manner, suggesting that Ac phy2 is the photoreceptor for fern spore germination. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect