Disrupting the cinnamyl alcohol dehydrogenase 1 gene (BdCAD1) leads to altered lignification and improved saccharification in Brachypodium distachyon

Autor: Wannes Voorend, Oumaya Bouchabke-Coussa, Madeleine Bouvier d’Yvoire, Caragh Whitehead, Simon J. McQueen-Mason, Richard Sibout, Lise Jouanin, Sébastien Antelme, Sylvain Legay, Philippe Lebris, Laurent Cézard, Catherine Lapierre, Leonardo D. Gomez, Frédéric Legée
Přispěvatelé: Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Plant Sci Unit Growth & Dev, Inst Agr & Fisheries Res, Ctr Novel Agr Prod, University of York [York, UK], RENEWALL, CELLWALL, ALICE French research project
Jazyk: angličtina
Rok vydání: 2013
Předmět:
0106 biological sciences
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences
DOWN-REGULATION
cinnamaldehydes
Sinapaldehyde
BICOLOR L. MOENCH
Cinnamyl-alcohol dehydrogenase
LIGNIN BIOSYNTHESIS
Plant Science
01 natural sciences
chemistry.chemical_compound
MOLECULAR-BASIS
Lignin
PHYLOGENETIC ANALYSIS
Phylogeny
0303 health sciences
education.field_of_study
Brachypodium distachyon
biology
food and beverages
Complementation
Biochemistry
Carbohydrate Metabolism
Brachypodium
Population
lignin
XYLEM EXPANSION
Genes
Plant
03 medical and health sciences
ARABIDOPSIS-HYPOCOTYL
Genetics
sinapic acid
education
Alleles
AGROBACTERIUM-MEDIATED TRANSFORMATION
030304 developmental biology
Alcohol dehydrogenase
cinnamyl alcohol dehydrogenase
BINARY VECTORS
Cell Biology
biology.organism_classification
saccharification
Alcohol Oxidoreductases
chemistry
Mutation
biology.protein
FERULIC ACID
010606 plant biology & botany
Zdroj: Plant Journal
Plant Journal, Wiley, 2013, 73 (3), pp.496-508. ⟨10.1111/tpj.12053⟩
ISSN: 0960-7412
1365-313X
DOI: 10.1111/tpj.12053⟩
Popis: Brachypodium distachyon (Brachypodium) has been proposed as a model for grasses, but there is limited knowledge regarding its lignins and no data on lignin-related mutants. The cinnamyl alcohol dehydrogenase (CAD) genes involved in lignification are promising targets to improve the cellulose-to-ethanol conversion process. Down-regulation of CAD often induces a reddish coloration of lignified tissues. Based on this observation, we screened a chemically induced population of Brachypodium mutants (Bd21-3 background) for red culm coloration. We identified two mutants (Bd4179 and Bd7591), with mutations in the BdCAD1 gene. The mature stems of these mutants displayed reduced CAD activity and lower lignin content. Their lignins were enriched in 8-O-4- and 4-O-5-coupled sinapaldehyde units, as well as resistant inter-unit bonds and free phenolic groups. By contrast, there was no increase in coniferaldehyde end groups. Moreover, the amount of sinapic acid ester-linked to cell walls was measured for the first time in a lignin-related CAD grass mutant. Functional complementation of the Bd4179 mutant with the wild-type BdCAD1 allele restored the wild-type phenotype and lignification. Saccharification assays revealed that Bd4179 and Bd7591 lines were more susceptible to enzymatic hydrolysis than wild-type plants. Here, we have demonstrated that BdCAD1 is involved in lignification of Brachypodium. We have shown that a single nucleotide change in BdCAD1 reduces the lignin level and increases the degree of branching of lignins through incorporation of sinapaldehyde. These changes make saccharification of cells walls pre-treated with alkaline easier without compromising plant growth.
Databáze: OpenAIRE
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