Proteomic analysis of Saccharomyces cerevisiae to study the effects of red wine polyphenols on oxidative stress
| Autor: | Daniel Alberto Wunderlin, María Verónica Baroni, Mariana S. Lingua, Carlos Batthyány, Ricardo Martin Neme Tauil |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Wine
0303 health sciences Antioxidant biology Chemistry medicine.medical_treatment Saccharomyces cerevisiae Hormesis food and beverages 04 agricultural and veterinary sciences biology.organism_classification Proteomics medicine.disease_cause 040401 food science 03 medical and health sciences 0404 agricultural biotechnology Biochemistry Proteome medicine French paradox Original Article Oxidative stress 030304 developmental biology Food Science |
| Zdroj: | J Food Sci Technol |
| Popis: | Understanding the molecular mechanisms underlying the “French paradox” has contributed to a growing interest in the investigation of the biological activity of red wine polyphenols (RWP). The main goal of this research is to provide valuable information on how RWP could exert their biological action at the cellular level. So, we report a proteomic analysis of S. cerevisiae exposed to both pro-oxidant (H(2)O(2)) and antioxidant (wine) agents. Cellular proteome analysis shows that RWP modify the level of certain proteins. Under both normal conditions (Wine treatment) and oxidative stress situations (Wine + H(2)O(2) treatment), the proteins involved in the metabolism and biosynthesis of biomolecules were down-regulated, while one ribosomal protein was up-regulated, probably performing its ribosome-independent functions, and so contributing to the stress defense system. Considering this action mechanism, we suggest that RWP may be acting as mild pro-oxidants and, therefore, exerting a hormetic effect that leads to the strengthening of cells’ antioxidant capacity. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s13197-019-03883-7) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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