Flax seed oil reduced tumor growth, modulated immune responses and decreased HPV E6 and E7 oncoprotein expression in a murine model of ectopic cervical cancer

Autor: Manjiri Karandikar, Ruchika Kaul-Ghanekar, Rashmi Deshpande, Prerna Raina, Prakash Mansara, Kavita Shinde
Rok vydání: 2019
Předmět:
0301 basic medicine
Linseed Oil
Antioxidant
Physiology
CD8 Antigens
Papillomavirus E7 Proteins
medicine.medical_treatment
Uterine Cervical Neoplasms
030204 cardiovascular system & hematology
Retinoblastoma Protein
Biochemistry
Antioxidants
Immunomodulation
Lipid peroxidation
Interferon-gamma
Mice
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Immune system
medicine
Animals
Humans
Cell Proliferation
Pharmacology
Cisplatin
Cervical cancer
biology
Chemistry
alpha-Linolenic acid
Fatty Acids
Oncogene Proteins
Viral
Cell Biology
medicine.disease
Xenograft Model Antitumor Assays
Gene Expression Regulation
Neoplastic
Repressor Proteins
Disease Models
Animal
030104 developmental biology
Apoptosis
Mitogen-activated protein kinase
Cancer research
biology.protein
Female
Interleukin-4
Lipid Peroxidation
Tumor Suppressor Protein p53
HeLa Cells
medicine.drug
Zdroj: Prostaglandins & Other Lipid Mediators. 143:106332
ISSN: 1098-8823
DOI: 10.1016/j.prostaglandins.2019.04.002
Popis: Cervical cancer is the second leading cause of cancer death in women in India. Previously, we have reported that alpha linolenic acid (ALA), induced apoptosis in cervical cancer cell lines and reduced expression of E6 and E7 oncoproteins with simultaneous decrease in Cox2/VEGF/MAP kinase proteins. Here, we investigated the tumor retardation potential of flax oil (FO), rich in ALA, in mouse papilloma model. Flax oil significantly reduced tumor volume and weight in mice compared to the Tumor control (TC) group. Interestingly, compared to cisplatin (Cis) alone, there was slightly enhanced decrease in tumor weight when FO was given together with Cis (Cis + FO). A marked increase in plasma antioxidant levels in mice, and increase in lipid peroxidation in tumors with simultaneous decrease in liver tissues was observed in Cis + FO group compared to either TC or Cis groups. FO and Cis + FO significantly modulated immune response in mice by increasing CD8α and IFNγ and decreasing IL-4 expression. Interestingly, when given together with cisplatin, flax oil reduced HPV E6 and E7 oncoprotein expression with concomitant increase in the relative mRNA expression of tumor suppressor genes p53 and Rb. Thus, flax oil could be explored for its therapeutic potential in cervical cancer.
Databáze: OpenAIRE
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