Interaction of toluene with polar stationary phases under conditions typical of hydrophilic interaction chromatography probed by saturation transfer difference nuclear magnetic resonance spectroscopy
| Autor: | Tobias Jonsson, Ngoc Phuoc Dinh, Muhammad Jamshaid Ashiq, Knut Irgum, Adel Shamshir, Tobias Sparrman |
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| Rok vydání: | 2019 |
| Předmět: |
Chromatography
Magnetic Resonance Spectroscopy Chemistry Hydrophilic interaction chromatography 010401 analytical chemistry Organic Chemistry Analytical chemistry Water General Medicine Nuclear magnetic resonance spectroscopy Acetates 010402 general chemistry 01 natural sciences Biochemistry Toluene 0104 chemical sciences Analytical Chemistry chemistry.chemical_compound Saturation transfer Void volume Polar Hydrophobic and Hydrophilic Interactions |
| Zdroj: | Journal of Chromatography A. 1588:58-67 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/j.chroma.2018.11.028 |
| Popis: | Toluene has been used as void volume (zero retention) marker since the inception of hydrophilic interaction chromatography (HILIC), based on the assumption that its hydrophobicity should prevent it from interacting with stationary phases envisioned to be covered by relatively thick layers of water. Recent work has shown that the void volumes of partly water-swollen HILIC phases are not identical to the volumes probed by toluene, yet the compound is still ubiquitously used as void volume marker. As part of our investigations of the retention mechanisms in HILIC, we probed the extent to which toluene is capable of penetrating into the water-enriched layer and to interact with the functional groups of three commercially available hydrophilic and polar stationary phases with different charge properties and water-retaining abilities, using saturation transfer difference |
| Databáze: | OpenAIRE |
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