Portal vein stenosis preconditioning of living donor liver in swine: early mechanisms of liver regeneration and gain of hepatic functional mass
| Autor: | Vincent Vidal, Théophile Guilbaud, Jean Hardwigsen, Eric Guedj, Christophe Buffat, Anaïs Palen, Guillaume Gorincour, Emilie Gregoire, Sophie Chopinet, Pauline Brige, Benjamin Guillet, Anaïs Moyon, Geraldine Hery |
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| Přispěvatelé: | Laboratoire d'Imagerie Interventionnelle Expérimentale (LIIE), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de pédiatrie multidisciplinaire [Hôpital de la Timone Enfants - APHM], Hôpital de la Timone [CHU - APHM] (TIMONE), Aix-Marseille Université - Faculté de médecine (AMU MED), Aix Marseille Université (AMU), Hôpital de la Conception [CHU - APHM] (LA CONCEPTION ), Aix-Marseille Université - Faculté de pharmacie (AMU PHARM), Centre recherche en CardioVasculaire et Nutrition (C2VN), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Service de Chirurgie, Assistance Publique - Hôpitaux de Marseille (APHM)-Hospices Civiles de Marseille-Hôpital de la Conception [CHU - APHM] (LA CONCEPTION ), Service Central de Biophysique et de Médecine Nucléaire, Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de Recherche en Informatique de Lens (CRIL), Université d'Artois (UA)-Centre National de la Recherche Scientifique (CNRS), Hôpital de la Conception [CHU - APHM] (LA CONCEPTION), Centre recherche en CardioVasculaire et Nutrition = Center for CardioVascular and Nutrition research (C2VN), Assistance Publique - Hôpitaux de Marseille (APHM)-Hospices Civiles de Marseille-Hôpital de la Conception [CHU - APHM] (LA CONCEPTION), Service de médecine nucléaire [Marseille], Université de la Méditerranée - Aix-Marseille 2-Assistance Publique - Hôpitaux de Marseille (APHM)- Hôpital de la Timone [CHU - APHM] (TIMONE), Imagerie MOléculaire pour applications THéranostiques personnalisées (IMOTHEP), Institut FRESNEL (FRESNEL), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Centre National de la Recherche Scientifique (CNRS)- Hôpital de la Timone [CHU - APHM] (TIMONE) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Models
Anatomic STAT3 Transcription Factor medicine.medical_specialty Swine Physiology medicine.medical_treatment Portal vein liver preconditioning Living donor 03 medical and health sciences Postoperative Complications 0302 clinical medicine Physiology (medical) Living Donors Animals Hepatectomy Medicine portal stenosis Ischemic Preconditioning liver regeneration Ligation ComputingMilieux_MISCELLANEOUS Hepatology Left portal vein Interleukin-6 Portal Vein Tumor Necrosis Factor-alpha business.industry Gastroenterology Organ Size Recovery of Function medicine.disease Peptide Fragments Liver regeneration Liver Transplantation major hepatectomy 3. Good health Surgery Stenosis Liver 030220 oncology & carcinogenesis Models Animal 030211 gastroenterology & hepatology LDLT business Major hepatectomy [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology |
| Zdroj: | AJP-Gastrointestinal and Liver Physiology AJP-Gastrointestinal and Liver Physiology, American Physiological Society, 2018, 315 (1), pp.G117-G125. ⟨10.1152/ajpgi.00390.2017⟩ AJP-Gastrointestinal and Liver Physiology, 2018, 315 (1), pp.G117-G125. ⟨10.1152/ajpgi.00390.2017⟩ |
| ISSN: | 0193-1857 1522-1547 |
| Popis: | To reduce the morbidity and mortality risk for the donor in living donor liver transplantation (LDLT), we previously identified 20% left portal vein (LPV) stenosis as an effective preconditioning method to induce cell proliferation in the contralateral lobe without downstream ipsilateral atrophy. In this study, we report the pathways involved in the first hours after preconditioning and investigate the changes in liver volume and function. Fourteen pigs were used this study. Five pigs were used to study the genetic, cellular and molecular mechanisms set up in the early hours following the establishment of our preconditioning. The remaining nine pigs were equally divided into three groups: sham-operated animals, 20% LPV stenosis, and 100% LPV stenosis. Volumetric scanning and 99 mTc-Mebrofenin hepatobiliary scintigraphy were performed before preconditioning and 14 days after to study morphological and functional changes in the liver. We demonstrated that liver regeneration triggered by 20% LPV stenosis in the contralateral lobe involves TNF-α, IL-6, and inducible nitric oxide synthase 2 by means of STAT3 and hepatocyte growth factor. We confirmed that our preconditioning was responsible for an increase in the total liver volume. Finally, we demonstrated that this volumetric gain was associated with an increase in hepatic functional capacity. NEW & NOTEWORTHY We describe a new preconditioning method for major hepatectomy that is applicable to hepatectomy for donation. We identified 20% left portal vein stenosis as effective preconditioning that is capable of inducing cell proliferation in the contralateral lobe without the downstream ipsilateral atrophy. In this study, we report the pathways involved in the first hours following preconditioning, and we confirm that 20% left portal vein stenosis is responsible for an increase in the functional capacity and total liver volume in a porcine model. |
| Databáze: | OpenAIRE |
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