The Effect of Gamma-irradiation on the Antibacterial Activity of Honey
| Autor: | Peter C. Molan, K L Allen |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Staphylococcus aureus
animal structures Pharmaceutical Science Microbial Sensitivity Tests Biology medicine.disease_cause Microbiology Wound Botulism medicine Food science Antibacterial agent Pharmacology digestive oral and skin physiology fungi Sterilization food and beverages Honey Hydrogen Peroxide Sterilization (microbiology) Clostridium perfringens Spore Wound Infection behavior and behavior mechanisms Clostridium botulinum Antibacterial activity |
| Zdroj: | Journal of Pharmacy and Pharmacology. 48:1206-1209 |
| ISSN: | 2042-7158 0022-3573 |
| DOI: | 10.1111/j.2042-7158.1996.tb03922.x |
| Popis: | There is increasing usage of honey as a dressing on infected wounds, burns and ulcers, but there is some concern that there may be a risk of wound botulism from the clostridial spores sometimes found in honey. It is well-established that the antibacterial activity is heat-labile so would be destroyed if honey were sterilized by autoclaving, but the effect of gamma-irradiation on the antibacterial activity of honey is not known. Therefore an investigation was carried out to assess the effect on the antibacterial activity of honey when the honey was subjected to a commercial sterilization procedure using gamma-irradiation (25 kGy). Two honeys with antibacterial activity due to enzymically-generated hydrogen peroxide and three manuka honeys with non-peroxide antibacterial activity were investigated. The honeys were tested against Staphylococcus aureus in an agar well diffusion assay. There was no significant change found in either type of antibacterial activity resulting from this form of sterilization of honey, even when the radiation was doubled (to 50 kGy). Testing of honey seeded with spores of Clostridium perfringens and C. tetani (10 000 and 1000 spores g−1 of honey, respectively) showed that 25 kGy of gamma-irradiation was sufficient to achieve sterility. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text