Endometrium On-a-Chip Reveals Insulin- and Glucose-induced Alterations in the Transcriptome and Proteomic Secretome
| Autor: | Haidee Tinning, Elton J R Vasconcelos, Tiago H C De Bem, Niamh Forde, Dapeng Wang |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Proteomics medicine.medical_specialty Stromal cell Proteome medicine.medical_treatment Primary Cell Culture Cell Culture Techniques microfluidics Embryonic Development Endometrium Transcriptome Andrology 03 medical and health sciences 0302 clinical medicine Endocrinology Pregnancy Internal medicine Lab-On-A-Chip Devices medicine Animals Insulin Secretion Cells Cultured Research Articles 030219 obstetrics & reproductive medicine Secretory Pathway uterus Chemistry Gene Expression Profiling bovine Embryo Embryo Mammalian Endometrium on-a-chip 030104 developmental biology medicine.anatomical_structure Glucose In utero cattle Female uterine luminal fluid AcademicSubjects/MED00250 |
| Zdroj: | Endocrinology |
| ISSN: | 1945-7170 |
| Popis: | The molecular interactions between the maternal environment and the developing embryo are key for early pregnancy success and are influenced by factors such as maternal metabolic status. Our understanding of the mechanism(s) through which these individual nutritional stressors alter endometrial function and the in utero environment for early pregnancy success is, however, limited. Here we report, for the first time, the use of an endometrium-on-a-chip microfluidics approach to produce a multicellular endometrium in vitro. Isolated endometrial cells (epithelial and stromal) from the uteri of nonpregnant cows in the early luteal phase (Days 4-7) were seeded in the upper chamber of the device (epithelial cells; 4-6 × 104 cells/mL) and stromal cells seeded in the lower chamber (1.5-2 × 104 cells/mL). Exposure of cells to different concentrations of glucose (0.5, 5.0, or 50 mM) or insulin (Vehicle, 1 or 10 ng/mL) was performed at a flow rate of 1 µL/minute for 72 hours. Quantitative differences in the cellular transcriptome and the secreted proteome of in vitro–derived uterine luminal fluid were determined by RNA-sequencing and tandem mass tagging mass spectrometry, respectively. High glucose concentrations altered 21 and 191 protein-coding genes in epithelial and stromal cells, respectively (P |
| Databáze: | OpenAIRE |
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