Sex determination by amplification of amelogenin gene from dental pulp tissue by polymerase Chain Reaction
| Autor: | Abhishek Singhvi, Surajit Goswami, Ripon Md Chowdhury, Sanya Bhatia, Neeta Bagul, Gurdeep Singh |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Male
Sex Determination Analysis sex determination Biology Environment Polymerase Chain Reaction law.invention 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine stomatognathic system law Humans Forensic odontology 030216 legal & forensic medicine General Dentistry Gene Polymerase chain reaction Amelogenin Gene Amplification 030206 dentistry General Medicine DNA Dna amplification Molecular biology environmental conditions lcsh:RK1-715 chemistry lcsh:Dentistry Salt water Nucleic acid Female dental pulp |
| Zdroj: | Indian Journal of Dental Research, Vol 29, Iss 4, Pp 470-476 (2018) |
| ISSN: | 1998-3603 0970-9290 |
| Popis: | Introduction: Forensic odontology necessarily involves the application of dentistry along with various other branches of sciences which deals with proper handling, examination, evaluation, and presentation of dental evidences, that aids to investigate a crime and deliver justice. Sex determination is a part of forensic odontology and an essential priority when traditional identification of the deceased becomes impossible. Aim: To determine Sex by analysis of the Amelogenin gene using Polymerase Chain Reaction (PCR) method on Deoxyribose nucleic acid (DNA) isolated from dental pulp, which was exposed to various environmental conditions created artificially to mimic a forensic scenario. Materials and Method: This in-vitro study was conducted by subjecting extracted teeth to various conditions imitating a forensic scene, viz. desiccation at room temperatures, immersion in salt water, burial in soil and even exposing to extremes of temperatures. DNA was extracted from dental pulp tissue and sex determination was achieved by amplification of the amelogenin gene through AMEL gene based primers in PCR. Result: Among all the samples used in this study, DNA could be extracted from all, except from those that were subjected to a temperature of 350 °C. DNA amplification and sex determination of the samples were found to be accurate when compared to sex of the individual which was recorded initially, during collection of teeth samples. Conclusion: This study shows teeth to be a potent source of DNA even in extreme environmental conditions, barring high temperatures and determination of sex by PCR amplification of AMEL markers to be quite reliable. |
| Databáze: | OpenAIRE |
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