1,25-Dihydroxyvitamin D3-induced growth restriction of cultured epithelial cells derived from a murine hepatic tumor
| Autor: | William Jubiz, Paul J. Higgins, Tanaka Yoko |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
medicine.medical_specialty
Biology Biochemistry Epithelium Mice Liver Neoplasms Experimental Calcitriol Internal medicine Tumor Cells Cultured Vitamin D and neurology medicine Animals Secretion Viability assay Pharmacology Cell growth Albumin Cell Differentiation Molecular biology Culture Media Chemically defined medium Endocrinology Mechanism of action Cell culture medicine.symptom Cell Division |
| Zdroj: | Biochemical Pharmacology. 38:449-453 |
| ISSN: | 0006-2952 |
| DOI: | 10.1016/0006-2952(89)90384-5 |
| Popis: | Recent evidence suggests that 1,25-dihydroxyvitamin D 3 can accumulate in certain presumed non-target tissues, although the mechanism of action of the vitamin in such cells is not understood. Exposure of 77-1/3a mouse hepatic tumor cells, which derived from a non-target tissue of vitamin D action, to 1,25-dihydroxyvitamin D 3 in chemically-defined serum-free medium resulted in a dose-dependent decline in cellular growth rate and maximal culture population density but did not adversely affect cell viability. Culture of 77-1/3a cells in defined medium containing 10 −7 or 10 −6 M 1,25-dihydroxyvitamin D 3 for 150 hr reduced the growth rate to 64 and 50% of control values respectively. Albumin secretion was unaffected by 1,25-dihydroxyvitamin D 3 exposure; in contrast, the cellular content of the proliferation-associated protein p35 was reduced by 39%, a decline similar in trend and degree to that observed in other tumor cells exposed to differentiation-inducing agents. It appears that 1,25-dihydroxyvitamin D 3 regulates cellular p35 content (within a specific restricted range) as a consequence of proliferative perturbation, rather than differentiated status, of cultured hepatic tumor cells. |
| Databáze: | OpenAIRE |
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