Replication of adenovirus type 5 DNA in KB cells: Localization and fate of parental DNA during replication
| Autor: | F. Spies, J.M. Vlak, Th.H. Rozijn |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
Cell Nucleus
DNA Replication biology viruses Eukaryotic DNA replication Molecular biology Adenoviridae Cell Line Proliferating cell nuclear antigen DNA replication factor CDT1 chemistry.chemical_compound Replication factor C medicine.anatomical_structure chemistry Control of chromosome duplication Virology DNA Viral biology.protein medicine Origin recognition complex Nucleus DNA |
| Zdroj: | Virology. 72:99-109 |
| ISSN: | 0042-6822 |
| DOI: | 10.1016/0042-6822(76)90315-9 |
| Popis: | The localization and fate of parental adenovirus type 5 (Ad5) DNA in KB cells at the time viral DNA replication occurs has been investigated by electron-microscope autoradiography and biochemical methods. At 18 hr after infection the majority of the parental Ad5 DNA molecules is present in the nucleus. About 60% of these molecules are involved in viral DNA replication by this time, but about 40% have not yet replicated at all. Electron-microscope autoradiography of cells infected with 3H-labeled Ad5 reveals that replicating parental Ad5 DNA molecules are randomly distributed throughout the nucleus, while nonreplicating viral DNA has a preference to locate in the periphery of the nucleus. Based on the experiments reported in this communication, the intranuclear fate of parental Ad5 DNA is discussed. |
| Databáze: | OpenAIRE |
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