Twice-daily red and blue light treatment for Candida albicans biofilm matrix development control

Autor: Malvin N. Janal, Beatriz Helena Dias Panariello, Iriana Carla Junqueira Zanin, Shane M. Maule, Cecilia Atem Gonçalves de Araújo Costa, Simone Duarte, Shawn M. Maule, Paula Ventura da Silveira
Rok vydání: 2018
Předmět:
Zdroj: Lasers in Medical Science. 34:441-447
ISSN: 1435-604X
0268-8921
DOI: 10.1007/s10103-018-2610-x
Popis: Phototherapy has been proposed as a direct means of affecting local bacterial infections. However, the use of phototherapy to prevent fungal biofilm development has received comparatively less attention. This study aimed to determine the effects of red light treatment and blue light treatment, without a photosensitizer, on the development of Candida albicans biofilm. During the development of 48-h biofilms of C. albicans SN 425 (n = 10), the biofilms were exposed twice-daily to noncoherent blue and red light (LumaCare; 420 nm and 635 nm). The energy density applied was 72 J cm−2 for blue light and 43.8 J cm2, 87.6 J cm2, and 175.5 J cm2 for red light. Positive control (PC) and negative control (NC) groups were treated twice-daily for 1 min with 0.12% chlorhexidine (CHX) and 0.89% NaCl respectively. Biofilms were analyzed for colony forming units (CFU), dry-weight, and exopolysaccharides (EPS-soluble and EPS-insoluble). Data was analyzed by one-way ANOVA and Tukey post hoc test (α = 0.05). Dry-weight was lower than NC (p
Databáze: OpenAIRE
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