miR156 switches on vegetative phase change under the regulation of redox signals in apple seedlings
| Autor: | Zhenhai Han, Xin Zhong Zhang, Xue Feng Xu, Qing Bo Zheng, Fei Shen, Ting Wu, Xiao Zhao Xu, Yi Wang, Zhen Du, Xiao Lin Jia, Yakun Chen |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Malus Transcription Genetic lcsh:Medicine Nicotiana benthamiana Genes Plant 01 natural sciences Article Vegetative phase change 03 medical and health sciences chemistry.chemical_compound Menadione Botany Transcriptional regulation lcsh:Science chemistry.chemical_classification Reactive oxygen species Multidisciplinary biology lcsh:R fungi Glutathione biology.organism_classification Cell biology MicroRNAs 030104 developmental biology chemistry Seedlings Glutathione disulfide lcsh:Q Reactive Oxygen Species Sugars Oxidation-Reduction Signal Transduction 010606 plant biology & botany |
| Zdroj: | Scientific Reports Scientific Reports, Vol 7, Iss 1, Pp 1-13 (2017) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-017-14671-8 |
| Popis: | In higher plants, miR156 regulates the vegetative phase change via the target SBP/SPL genes. The regulation of miR156 during ontogenetic processes is not fully understood. In the apple genome, of 31 putative MdMIR156 genes that encode pre-miR156, seven were dominantly expressed. However, the transcript levels of only MdMIR156a5 and MdMIR156a12 decreased significantly during the vegetative phase change, which was consistent with the mature miR156 level, indicating that miR156 is under transcriptional regulation. Leaf H2O2 content was higher in the adult phase than in the juvenile phase because of excess H2O2 accumulation in chloroplasts. When in vitro shoots were treated with menadione, diphenyleneiodonium, L-2-oxothiazolidine-4-carboxylic acid or buthionine sulphoximine, the expressions of MdMIR156a5, MdMIR156a12, and as well miR156 were coordinated with reduced glutathione (GSH) contents and glutathione/glutathione disulfide ratio but not H2O2 contents. Alteration of miR156 expression level by MdMIR156a6-overexpressing or miR156-mimetic transgenic Nicotiana benthamiana did not cause a corresponding change in reactive oxygen species or GSH status. Collectively, the results indicate that the vegetative phase change in apple is controlled by the MdMIR156a5 and MdMIR156a12 transcriptional regulatory network in response to the plastid–nucleus redox signals, such as GSH. |
| Databáze: | OpenAIRE |
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