Fabricating Covalent Organic Framework Capsules with Commodious Microenvironment for Enzymes
| Autor: | Xia Li, Zhenjie Zhang, Yassin H. Andaloussi, Ang Li, Shengqian Ma, Shan Qiao, Yao Chen, Peng Cheng, Mingmin Li, Yunlong Zheng |
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| Přispěvatelé: | National Natural Science Foundation of China, Tianjin Natural Science Foundation of China |
| Rok vydání: | 2020 |
| Předmět: |
chemistry.chemical_classification
Immobilized enzyme Chemistry Nanotechnology General Chemistry 010402 general chemistry Enzymes Immobilized 01 natural sciences Biochemistry Catalysis 0104 chemical sciences enzyme activity Colloid and Surface Chemistry Enzyme Metal-Organic Frameworks Covalent organic framework |
| Zdroj: | Journal of the American Chemical Society. 142(14) |
| ISSN: | 1520-5126 |
| Popis: | Enzyme immobilization has been demonstrated to be a favorable protocol to promote industrialization of biomacromolecules. Despite tremendous efforts to develop new strategies and materials to realize this process, maintaining enzyme activity is still a formidable challenge. Herein we created a sacrificial templating method, using metal-organic frameworks (MOFs) as sacrificial templates to construct hollow covalent organic framework (COF) capsules for enzyme encapsulation. This strategy can provide a capacious microenvironment to unleash enzyme molecules. The improved conformational freedom of enzymes, enhanced mass transfer, and protective effect against the external environment ultimately boosted the enzymatic activities. We also found that this strategy possesses high versatility that is suitable for diverse biomacromolecules, MOF templates, and COF capsules. Moreover, the dimensions, pore sizes, and shell thickness of COF capsules can be conveniently tuned, allowing for customizing bioreactors for specific functions. For example, coencapsulation of different enzymes with synergistic functions were successfully demonstrated using this bioreactor platform. This study not only opens up a new avenue to overcome the present limitations of enzymatic immobilization in porous matrixes but also provides new opportunities for construction of biomicrodevices or artificial organelles based on crystalline porous materials. |
| Databáze: | OpenAIRE |
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