Top-Down Garbage Collector: a tool for selecting high-quality top-down proteomics mass spectra
| Autor: | Julia Chamot-Rooke, Diogo B. Lima, André R.F. Silva, Valmir C. Barbosa, Milan A. Clasen, Marlon D.M. Santos, Mathieu Dupré, Louise U. Kurt, Paulo C. Carvalho, Priscila F Aquino |
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| Přispěvatelé: | Spectrométrie de Masse pour la Biologie – Mass Spectrometry for Biology (UTechS MSBio), Institut Pasteur [Paris]-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Fiocruz Paraná - Instituto Carlos Chagas / Carlos Chagas Institute [Curitiba, Brésil] (ICC), Fundação Oswaldo Cruz (FIOCRUZ), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Instituto Leônidas e Maria Deane - Fiocruz Amazônia [Manaus, Brésil] (ILMD), Universidade Federal do Rio de Janeiro (UFRJ), This work has been supported by Fiocruz, the Brazilian National Research Council (CNPq) and Graduate Studies Agency (CAPES), a FAPERJ BBP grant and an Agence Nationale de la Recherche (project ANR-15-CE18-0021) grant., ANR-15-CE18-0021,PathoTOP,Identification rapide de pathogènes bactériens par protéomique top-down en contexte clinique(2015), Institut Pasteur [Paris] (IP)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Fundação Oswaldo Cruz / Oswaldo Cruz Foundation (FIOCRUZ) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Proteomics
Statistics and Probability Computer science media_common.quotation_subject [SDV]Life Sciences [q-bio] computer.software_genre Top-down proteomics Biochemistry 03 medical and health sciences Tandem Mass Spectrometry Escherichia coli Quality (business) [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Molecular Biology 030304 developmental biology media_common 0303 health sciences 030302 biochemistry & molecular biology Top-down and bottom-up design Computer Science Applications Computational Mathematics Identification (information) Computational Theory and Mathematics Mass spectrum Data mining [INFO.INFO-BI]Computer Science [cs]/Bioinformatics [q-bio.QM] computer Software Garbage collection |
| Zdroj: | Bioinformatics Bioinformatics, Oxford University Press (OUP), 2019, 35 (18), pp.3489-3490. ⟨10.1093/bioinformatics/btz085⟩ Bioinformatics, 2019, 35 (18), pp.3489-3490. ⟨10.1093/bioinformatics/btz085⟩ |
| ISSN: | 1367-4803 1367-4811 |
| DOI: | 10.1093/bioinformatics/btz085⟩ |
| Popis: | Motivation We present the first tool for unbiased quality control of top-down proteomics datasets. Our tool can select high-quality top-down proteomics spectra, serve as a gateway for building top-down spectral libraries and, ultimately, improve identification rates. Results We demonstrate that a twofold rate increase for two E. coli top-down proteomics datasets may be achievable. Availability and implementation http://patternlabforproteomics.org/tdgc, freely available for academic use. Supplementary information Supplementary data are available at Bioinformatics online. |
| Databáze: | OpenAIRE |
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