Nuclear Remodeling in Bovine Somatic Cell Nuclear Transfer Embryos Using MG132-Treated Recipient Oocytes
| Autor: | Ribeiro-Mason, Karlla, Boulesteix, Claire, Fleurot, Renaud, Aguirre-Lavin, Tiphaine, Adenot, Pierre, Debey, Pascale, Le Bourhis, Daniel, Beaujean, Nathalie, Ruffini, Sylvie, Vignon, Xavier, Gall, Laurence |
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| Přispěvatelé: | Biologie du Développement et Reproduction (BDR), Institut National de la Recherche Agronomique (INRA), Biologie du développement et reproduction (BDR), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA) |
| Rok vydání: | 2010 |
| Předmět: |
Nuclear Transfer Techniques
Leupeptins Cloning Organism [SDV]Life Sciences [q-bio] Maturation-Promoting Factor Cyclin B Embryonic Development macromolecular substances [SDV.BC]Life Sciences [q-bio]/Cellular Biology Cysteine Proteinase Inhibitors 03 medical and health sciences 0302 clinical medicine medicine Animals BIOLOGIE DU DEVELOPPEMENT Blastocyst immunofluorescence Kinase activity SOMATIC CELL NUCLEAR TRANSFER [SDV.BDD]Life Sciences [q-bio]/Development Biology Cells Cultured ComputingMilieux_MISCELLANEOUS 030304 developmental biology Cell Nucleus 0303 health sciences 030219 obstetrics & reproductive medicine biology EMBRYO [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology Embryo Cell Biology Fibroblasts Cellular Reprogramming Embryo Mammalian Oocyte Molecular biology Chromatin Embryo transfer Cell biology medicine.anatomical_structure Premature chromosome condensation Oocytes biology.protein Somatic cell nuclear transfer Cattle Developmental Biology Biotechnology |
| Zdroj: | CELLULAR REPROGRAMMING CELLULAR REPROGRAMMING, 2010, 12 (6), pp.729-738. ⟨10.1089/cell.2010.0035⟩ |
| ISSN: | 2152-4998 2152-4971 |
| DOI: | 10.1089/cell.2010.0035 |
| Popis: | International audience; The early events in the nuclear reprogramming process during somatic cell nuclear transfer (SCNT) consist of morphological remodeling of the donor nucleus including premature chromosome condensation (PCC). In the present study, the objective was to increase oocyte M-Phase Promoting Factor (MPF) kinase activity and to examine the fate of the donor nucleus and the development of SCNT embryos thereafter. Indeed, in controls, recipient oocytes activated upon nuclear transfer, undergo a decrease in MPF activity, responsible for the inability to promote PCC in 77.8% of reconstituted embryos. Here we showed that exposure of the recipient oocyte to the proteasome inhibitor MG132 prior to fusion inhibited the degradation of cyclin B, which normally occurred immediately after activation by electro stimulation, and therefore sustained a high level of MPF. Treatment with MG132 also significantly increased the percentage of SCNT embryos with PCC when compared to the nontreated SCNT control embryos (94.1 vs. 22.2%, respectively, p |
| Databáze: | OpenAIRE |
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