| Autor: |
Jiri Novacek, Pavel Veverka, Tomas Brom, Matyas Pinkas, Ctirad Hofr, Tomáš Janovič, Martin Stojaspal |
| Rok vydání: |
2022 |
| Předmět: |
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| Popis: |
The RE1-Silencing Transcription factor (REST) is essential for neuronal differentiation. Here, we report the first 18.5-angstrom electron microscopy structure of human REST. The refined electron map suggests that REST forms a torus that can accommodate DNA double-helix in the central hole. Additionally, we quantitatively described REST binding to the canonical DNA sequence of the neuron-restrictive silencer element. We developed protocols for the expression and purification of full-length REST and the shortened variant REST-N62 produced by alternative splicing. We tested the mutual interaction of full-length REST and the splicing variant REST-N62. Revealed structure-function relationships of master neuronal repressor REST will allow finding new biological ways of prevention and treatment of neurodegenerative disorders and diseases. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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