Development of a perfusion reversed-phase HPLC method for the separation of soybean and cereal (wheat, corn, and rice) proteins in binary mixtures. Application to the detection of soybean proteins in commercial bakery products
| Autor: | María Concepción García, María Luisa Marina, Florentina Castro-Rubio, A. Castro-Rubio |
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| Rok vydání: | 2005 |
| Předmět: |
Chromatography
Extraction (chemistry) food and beverages Filtration and Separation Reversed-phase chromatography Bread High-performance liquid chromatography Analytical Chemistry Acetic acid chemistry.chemical_compound chemistry Solubility Plant protein Trifluoroacetic acid Soybean Proteins Edible Grain Soy protein Chromatography High Pressure Liquid Plant Proteins |
| Zdroj: | Journal of separation science. 28(9-10) |
| ISSN: | 1615-9306 |
| Popis: | A perfusion reversed-phase HPLC method enabling the simultaneous separation of soybean and cereal (wheat, corn, and rice) proteins in commercial bakery products has been proposed for the first time. The method utilises an acetonitrile-water gradient containing an ion-pairing agent. Different ion-pairing agents were tried, 0.3% (v/v) acetic acid being observed to enable the separation of soybean from wheat, rice, and corn proteins while with 0.1% (v/v) trifluoroacetic acid only the separation of soybean and corn proteins was possible. Optimisation of the solubilisation conditions for proteins was achieved by testing different acetonitrile concentrations for the simultaneous extraction of soybean and cereal proteins: best recoveries were found with 25% (v/v) acetonitrile + 0.3% (v/v) acetic acid and with 40% (v/v) acetonitrile + 0.1% (v/v) trifluoroacetic acid. Chromatographic conditions such as gradient, temperature, and wavelength detection were also optimised. The method enabled the separation of soybean and cereal proteins in binary mixtures (soybean and wheat, soybean and corn, or soybean and rice proteins) in less than 5 minutes in a total analysis time of 20 min. |
| Databáze: | OpenAIRE |
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