Lactobacillus plantarum surface-displayed ASFV (p54) with porcine IL-21 generally stimulates protective immune responses in mice
| Autor: | Nan Wang, Jian-Zhong Wang, Chun-Wei Shi, Chun-Feng Wang, Xin Cao, Wei Zhao, Jun-Hong Wang, Hai-Bin Huang, Gui-Lian Yang, Kai-Dian Yang, Wen-Tao Yang, Tian-Ming Niu, Yanlong Jiang, Yan Zeng, Xiao-Lei Chen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
p54
Biophysics Lymphocyte proliferation Applied Microbiology and Biotechnology Microbiology Virus Flow cytometry Immune system medicine Cytotoxic T cell Immune evaluation Recombinant biology medicine.diagnostic_test food and beverages biology.organism_classification Fusion protein QR1-502 Humoral immunity bacteria L. plantarum Original Article ASFV Lactobacillus plantarum TP248.13-248.65 Biotechnology |
| Zdroj: | AMB Express, Vol 11, Iss 1, Pp 1-11 (2021) AMB Express |
| ISSN: | 2191-0855 |
| Popis: | African classical swine fever virus (ASFV) has spread seriously around the world and has dealt with a heavy blow to the pig breeding industry due to the lack of vaccines. In this study, we produced recombinant Lactobacillus plantarum (L. plantarum) expressing an ASFV p54 and porcine IL-21 (pIL-21) fusion protein and evaluated the immune effect of NC8-pSIP409-pgsA'-p54-pIL-21 in a mouse model. First, we verified that the ASFV p54 protein and p54-pIL-21 fusion protein were anchored on the surface of L. plantarum NC8 by flow cytometry, immunofluorescence and Western blotting. Then, the results were verified by flow cytometry, ELISA and MTT assays. Mouse-specific humoral immunity and mucosal and T cell-mediated immune responses were induced by recombinant L. plantarum. The results of feeding mice recombinant L. plantarum showed that the levels of serum IgG and mucosal secreted IgA (SIgA), the number of CD4 and CD8 T cells, and the expression of IFN-γ in CD4 and CD8 T cells increased significantly, and lymphocyte proliferation occurred under stimulation with the ASFV p54 protein. Our data lay a foundation for the development of oral vaccines against ASFV in the future. |
| Databáze: | OpenAIRE |
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