A model for testing the mutagenicity of excimer laser radiation in ophthalmology
| Autor: | Werner Förster, J. Weber, W. Scheid, H. Traut |
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| Rok vydání: | 2009 |
| Předmět: |
Adult
medicine.medical_specialty Anterior Chamber medicine.medical_treatment Chromatids Radiation Eye law.invention Cornea Corneal surgery law Ophthalmology medicine Animals Humans Lymphocytes Irradiation Whole blood Chromosome Aberrations Excimer laser Mutagenicity Tests Chemistry Lasers food and beverages General Medicine Laser Disease Models Animal medicine.anatomical_structure Cattle Female Chromatid |
| Zdroj: | Acta Ophthalmologica. 69:533-535 |
| ISSN: | 1755-375X |
| Popis: | Because excimer laser radiation is applied more and more in therapeutic and refractive corneal surgery, the possibility of mutagenic side effects of this treatment should be considered. A mutagenicity model is presented approximating closely the ophthalmological conditions when mutagenic effects are studied: Heparinized human blood is filled into the anterior chamber of an enucleated cow eye. After irradiation of the cornea with the excimer laser, the blood is removed and whole blood cultures are set up. Lymphocytes are stimulated, fixed during metaphase, and scored for chromatid and chromosome aberrations. First results obtained with this model after 248 nm laser irradiation (500 J, 277 mJ/cm2, 10 or 20 Hz) revealed no mutagenic effects. However, when the blood was exposed under the same physical conditions in a quartz chamber (positive control), statistically highly significant increases of the yields of chromatid aberrations were observed. |
| Databáze: | OpenAIRE |
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