Role of the Fas System in Liver Regeneration after a Partial Hepatectomy in Rats
| Autor: | S. Hiroyasu, Kaoru Taira, Masayuki Shiraishi, Takehiko Koji, Yoshihiro Muto |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Male
Pathology medicine.medical_specialty Fas Ligand Protein medicine.medical_treatment Apoptosis Fas ligand Andrology In vivo In Situ Nick-End Labeling medicine Animals Hepatectomy fas Receptor Rats Wistar Membrane Glycoproteins TUNEL assay biology Reverse Transcriptase Polymerase Chain Reaction Immunohistochemistry Liver regeneration Liver Regeneration Rats Proliferating cell nuclear antigen Liver Proto-Oncogene Proteins c-bcl-2 biology.protein Surgery |
| Zdroj: | European Surgical Research. 33:334-341 |
| ISSN: | 1421-9921 0014-312X |
| DOI: | 10.1159/000049727 |
| Popis: | Apoptosis is involved in the homeostatic control of organs. The aim of this study was to define the in vivo role of apoptosis-related proteins including the Fas system and Bcl-2 in liver regeneration following a partial hepatectomy (PH). We used 70% hepatectomized rats which were serially sacrificed from 12 h to 28 days. The expressions of Fas, Fas ligand, and Bcl-2 were examined by semiquantitative RT-PCR and immunohistochemistry. Liver regeneration, as examined by PCNA staining, peaked from 24 h to day 3, and declined from day 5. On the other hand, hepatocyte apoptosis, as examined by TUNEL staining, was seldom observed until 24 h, but increased from 1 week after PH. In the RT-PCR study, Fas showed an early decline by 24 h, followed by a later peak from days 3 to 5, and then a constant expression thereafter. Meanwhile, the Fas ligand was also low until day 3, but showed a remarkable increase from days 5 to 7, followed by a gradual decrease. On the other hand, Bcl-2 showed an early peak until 24 h, followed by a decline from day 5. In an immunohistochemical study, the time courses of these protein expressions were almost synchronous with their mRNAs in the RT-PCR study. We thus conclude that the coordinated interplay between these apoptosis-related proteins and hepatocyte apoptosis suggests the possible involvement of these proteins in the course of liver regeneration. |
| Databáze: | OpenAIRE |
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