Survival, excision repair and inhibition of DNA synthesis in normal human skin fibroblasts exposed to simulated sunlight
| Autor: | Barry S. Rosenstein, Rebecca B. Rosenstein |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Adult
DNA Replication Time Factors DNA Repair Cell Survival DNA repair Biophysics Human skin Biology Cell Line chemistry.chemical_compound medicine Humans Radiology Nuclear Medicine and imaging Irradiation Fibroblast Skin Genetics Sunlight Photolysis Radiation integumentary system Radiological and Ultrasound Technology DNA synthesis Dose-Response Relationship Radiation DNA Fibroblasts Molecular biology Kinetics medicine.anatomical_structure Bromodeoxyuridine chemistry Female DNA Damage Nucleotide excision repair |
| Zdroj: | Journal of Photochemistry and Photobiology B: Biology. 16:247-255 |
| ISSN: | 1011-1344 |
| DOI: | 10.1016/1011-1344(92)80013-l |
| Popis: | The responses of normal human skin fibroblasts exposed to simulated sunlight produced by a solar simulator were examined. The parameters investigated were cellular survival, excision repair and the inhibition and recovery of DNA synthesis. The latter two effects were examined using the bromodeoxyuridine photolysis assay and the alkaline step elution assay respectively. The results of these experiments are consistent with the conclusion that the lesions induced by simulated sunlight represent a mixture of damage which elicits cellular responses and repair mechanisms similar to those manifested by cells irradiated with UVC and UVA radiation. |
| Databáze: | OpenAIRE |
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