The Hippocamposeptal Pathway Generates Rhythmic Firing of GABAergic Neurons in the Medial Septum and Diagonal Bands: An Investigation Using a Complete Septohippocampal Preparation In Vitro
| Autor: | Romain Goutagny, Marc Danik, Sylvain Williams, Frédéric Manseau |
|---|---|
| Přispěvatelé: | Douglas Mental Health University Institute [Montréal], McGill University = Université McGill [Montréal, Canada], Laboratoire de neurosciences cognitives et adaptatives (LNCA), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Periodicity
MESH: Periodicity MESH: Hippocampus [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology MESH: Neurons Hippocampus Action Potentials MESH: Rats Sprague-Dawley MESH: gamma-Aminobutyric Acid Hippocampal formation Diagonal Band of Broca Synaptic Transmission MESH: Animals Newborn Rats Sprague-Dawley 0302 clinical medicine Rhythmic inhibition Neural Pathways MESH: Animals MESH: Oscillometry gamma-Aminobutyric Acid MESH: Action Potentials MESH: Inhibitory Postsynaptic Potentials Neurons 0303 health sciences MESH: Electrophysiology General Neuroscience [SDV.BA]Life Sciences [q-bio]/Animal biology Fornix MESH: Electric Stimulation MESH: Neural Inhibition Articles Electrophysiology MESH: Cholinergic Agonists GABAergic [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] MESH: Rats MESH: Diagonal Band of Broca Biology Cholinergic Agonists In Vitro Techniques 03 medical and health sciences Glutamatergic Oscillometry Reaction Time MESH: Synaptic Transmission Animals 030304 developmental biology MESH: In Vitro Techniques MESH: Neural Pathways Neural Inhibition Electric Stimulation Rats MESH: Reaction Time MESH: Carbachol Animals Newborn Inhibitory Postsynaptic Potentials nervous system Cholinergic Carbachol Septal Nuclei MESH: Septal Nuclei Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Journal of Neuroscience Journal of Neuroscience, Society for Neuroscience, 2008, 28 (15), pp.4096-4107. ⟨10.1523/JNEUROSCI.0247-08.2008⟩ |
| ISSN: | 0270-6474 1529-2401 |
| Popis: | The medial septum diagonal band area (MS/DB) projects to the hippocampus through the fornix/fimbria pathway and is implicated in generating hippocampal theta oscillations. The hippocampus also projects back to the MS/DB, but very little is known functionally about this input. Here, we investigated the physiological role of hippocamposeptal feedback to the MS/DB in a completein vitroseptohippocampal preparation containing the intact interconnecting fornix/fimbria pathway. We demonstrated that carbachol-induced rhythmic theta-like hippocampal oscillations recorded extracellularly were synchronized with powerful rhythmic IPSPs in whole-cell recorded MS/DB neurons. Interestingly, we found that these IPSPs evoked rebound spiking in GABAergic MS/DB neurons. In contrast, putative cholinergic and glutamatergic MS/DB neurons responded only weakly with rebound spiking and, as a result, were mostly silent during theta-like oscillations. We next determined the mechanism underlying the rebound spiking that followed the IPSPs in MS/DB GABAergic neurons using phasic electrical stimulation of the fornix/fimbria pathway. We demonstrate that the increased rebound spiking was attributable to the activation ofIhcurrent, because it was significantly reduced by low concentrations of theIhantagonist ZD7288 [4-(N-ethyl-N-phenylamino)-1,2-dimethyl-6-(methylamino) pyridinium chloride]. Together, these results suggest that rhythmical activity in hippocampus is transferred to the MS/DB and can preferentially phase the spiking of GABAergic MS/DB neurons because of their significant expression ofIhcurrents. Our data demonstrate that hippocamposeptal inhibition facilitates theta rhythmic discharges in MS/DB GABAergic neurons while favoring the inhibition of most ACh and glutamate neurons. |
| Databáze: | OpenAIRE |
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