Intrinsic and extrinsic factors that may influence DNA preservation in skeletal remains: A review
| Autor: | Francisco Etxeberria, Caterina Raffone, Nicole Lambacher, Marian M. de Pancorbo, Miriam Baeta, Eva Granizo-Rodríguez |
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| Rok vydání: | 2020 |
| Předmět: |
Biology
01 natural sciences Bone and Bones Pathology and Forensic Medicine 03 medical and health sciences chemistry.chemical_compound Soil 0302 clinical medicine DNA degradation Bone Density Humans 030216 legal & forensic medicine Groundwater Soil Microbiology 010401 analytical chemistry DNA Degradation Necrotic Temperature Humidity DNA Forensic Medicine Hydrogen-Ion Concentration DNA Fingerprinting 0104 chemical sciences Body Remains chemistry Evolutionary biology Postmortem Changes Identification (biology) Collagen Law Tooth |
| Zdroj: | Forensic science international. 325 |
| ISSN: | 1872-6283 |
| Popis: | The identification of skeletal human remains, severely compromised by putrefaction, or highly deteriorated, is important for legal and humanitarian reasons. There are different tools that can help in the identification process such as anthropological and genetic studies. The success observed during the last decade in genetic analysis of skeletal remains has been possible especially due to the refinements of DNA extraction and posterior analysis techniques. However, despite these progresses, many challenges keep influencing the results of such analysis, mainly the limited amount and the degradation of the DNA recovered from badly preserved samples. By now, there is still no wide-range knowledge about post-mortem kinetics of DNA degradation. Therefore, taphonomy studies can play a key role in the reconstruction of post-mortem transformations that skeletal remains, and consequently DNA, have undergone. Thus, the goal of the present review focuses on the assessment of the literature regarding the possible effect of intrinsic (characteristics of the bone) and extrinsic (environmental) factors on the state of preservation of skeletal remains recovered in a terrestrial environment and their genetic material. The establishment of useful indicators describing the state of the remains is a key factor in order to determine their suitability for posterior biomolecular analysis. |
| Databáze: | OpenAIRE |
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