High-performance liquid chromatographic separation of stereoisomers of N-phthaloyl-protected amino acids and dipeptidomimetics
| Autor: | Antal Péter, István Ilisz, Karolien Van Rompaey, Rien De Wachter, Dirk Tourwé, Steven Ballet, Daniel W. Armstrong |
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| Rok vydání: | 2007 |
| Předmět: |
Ultraviolet Rays
Filtration and Separation Peptide High-performance liquid chromatography Chemistry Techniques Analytical Mass Spectrometry Analytical Chemistry chemistry.chemical_compound Organic chemistry Amino Acids Derivatization Chromatography High Pressure Liquid chemistry.chemical_classification Chromatography Dipeptide Bicyclic molecule Molecular Structure Chemistry Elution Glycopeptides Reproducibility of Results Stereoisomerism Hydrogen-Ion Concentration Amino acid Models Chemical Enantiomer Peptides |
| Zdroj: | Journal of separation science. 30(12) |
| ISSN: | 1615-9306 |
| Popis: | The stereoisomers of N-phthaloyl-protected amino acids and dipeptidomimetics were separated on macrocyclic glycopeptide and cellulose-based chiral stationary phases (CSPs) in the RP and polar-ionic modes. The effects of the organic modifier, the mobile phase composition, and the pH on the separations were investigated. Optimization of these separations was achieved through variation of the mobile-phase additive combinations. The elution sequence was determined for some of the samples. A practical application for the monitoring of the reaction conditions for N-phthaloylation of (S)-Phe was demonstrated. |
| Databáze: | OpenAIRE |
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