Isolation and identification of genes activating UAS2-dependent ADH2 expression in Saccharomyces cerevisiae
| Autor: | Michael S. Donoviel, Elton T. Young |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
DNA
Complementary Saccharomyces cerevisiae Proteins Recombinant Fusion Proteins Molecular Sequence Data Gene Dosage Saccharomyces cerevisiae Biology Regulatory Sequences Nucleic Acid Investigations Gene dosage Gene Expression Regulation Enzymologic Frameshift mutation Fungal Proteins Genes Reporter Gene Expression Regulation Fungal Gene expression Genetics Histone Chaperones Enhancer DNA Fungal Gene Aldose-Ketose Isomerases Regulation of gene expression Base Sequence Alcohol Dehydrogenase Nuclear Proteins beta-Galactosidase Fusion protein Open reading frame Glucose Trans-Activators Transcriptional Elongation Factors |
| Zdroj: | Genetics. 143(3) |
| ISSN: | 0016-6731 |
| Popis: | Two cis-acting elements have been identified that act synergistically to regulate expression of the glucose-repressed alcohol dehydrogenase 2 (ADH2) gene. UAS1 is bound by the trans-activator Adrlp. UAS2 is thought to be the binding site for an unidentified regulatory protein. A genetic selection based on a UAS2-dependent ADH2 reporter was devised to isolate genes capable of activating UAS2-dependent transcription. One set of UAS2-dependent genes contained SPT6/CRE2/SSN20. Multicopy SPT6 caused improper expression of chromosomal ADH2. A second set of UAS2-dependent clones contained a previously uncharacterized open reading frame designated MEU1 (Multicopy Enhancer of UAS2). A frame shift mutation in MEU1 abolished its ability to activate UAS2-dependent gene expression. Multicopy MEU1 expression suppressed the constitutive ADH2 expression caused by cre2-1. Disruption of MEU1 reduced endogenous ADH2 expression about twofold but had no effect on cell viability or growth. No homologues of MEU1 were identified by low-stringency Southern hybridization of yeast genomic DNA, and no significant homologues were found in the sequence data bases. A MEU1/β-gal fusion protein was not localized to a particular region of the cell. MEU1 is linked to PPR1 on chromosome XII. |
| Databáze: | OpenAIRE |
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