Genome rearrangements in host-range mutants of the polyvalent staphylococcal bacteriophage 812
| Autor: | Petr Kašpárek, Roman Pantůček, Jiří Doškař, Vladislava Růžičková, Jana Kahánková |
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| Rok vydání: | 2007 |
| Předmět: |
Staphylococcus aureus
Molecular Sequence Data Mutant Lysin Genome Viral Biology Microbiology Genome rearrangement Genome Bacteriophage 03 medical and health sciences Endopeptidases Bacteriophages Amino Acid Sequence Gene 030304 developmental biology Genetics 0303 health sciences Base Sequence Reverse Transcriptase Polymerase Chain Reaction 030306 microbiology Intron Viral Tail Proteins General Medicine biology.organism_classification Molecular biology Bacteriophage Therapy Mutation RNA Viral Sequence Alignment Polymorphism Restriction Fragment Length |
| Zdroj: | Folia Microbiologica. 52:331-338 |
| ISSN: | 1874-9356 0015-5632 |
| Popis: | Mutations extended the host range of the polyvalent bacteriophage 812 of the family Myoviridae in up to 95 % of Staphylococcus aureus strains and 43 % of strains of different coagulase-positive and -negative Staphylococcus species. Mutational changes in the genome of several host-range mutants of phage 812 were identified. Host-range mutant 812F1 harbors a deletion in endolysin gene that arose together with intron excision. Four mutants (812i, 812b, 812p, 812F3) harbor deletion in the structural gene orf8 that results from a genome rearrangement associated with intron insertion. This rearrangement was also detected in the genome of the closely related phages U16 and phi131. Another intron was discovered in the recA812 gene in these four mutants. An insertion was found in a non-coding region of the restriction fragment PstI-O of three mutants (812b, 812F3, 812g) and phages U16 and phi131. The above results contribute to the explanation of genetic factors affecting the host range of polyvalent staphylococcal bacteriophages. |
| Databáze: | OpenAIRE |
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