Bacillomycin D and its combination with amphotericin B: promising antifungal compounds with powerful antibiofilm activity and wound-healing potency
| Autor: | Bruno Casciaro, I. Ben Slimene, A. Di Grazia, Sana Azaiez, Ines Karkouch, Ferid Limam, Maria Luisa Mangoni, Vincenzo Luca, Olfa Tabbene, M. N. Alfeddy, Salem Elkahoui |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
amphotericin b anti-biofilm bacillomycin d candida albicans synergy wound healing applied microbiology and biotechnology biotechnology Antifungal Agents 030106 microbiology Antifungal drug Germ tube Microbial Sensitivity Tests Pharmacology Biology Applied Microbiology and Biotechnology Microbiology 03 medical and health sciences chemistry.chemical_compound Amphotericin B Candida albicans medicine Humans Potency Wound Healing Candidiasis Biofilm Lipopeptide General Medicine biology.organism_classification Corpus albicans 030104 developmental biology chemistry Biofilms Drug Therapy Combination Peptides Antimicrobial Cationic Peptides Biotechnology medicine.drug |
| Zdroj: | Journal of Applied Microbiology. 120:289-300 |
| ISSN: | 1364-5072 |
| DOI: | 10.1111/jam.13030 |
| Popis: | AIMS In this study, we evaluated the ability of the lipopeptide bacillomycin D and the antifungal drug amphotericin B as well as their combination, to inhibit Candida albicans biofilm formation and to accelerate keratinocyte cell migration. METHODS AND RESULTS The antibiofilm activity of bacillomycin D and its combination with amphotericin B was carried out by crystal violet colorimetric method. Our results have shown that, when combined together at low concentrations nontoxic to mammalian cells, corresponding to 1/32 MIC (0·39 μg ml(-1) ) and 1/4 MIC (0·06 μg ml(-1) ) for bacillomycin D and amphotericin B, respectively, a clear antibiofilm activity is manifested (95% inhibition of biofilm formation) along with a clear inhibition of germ tube formation. Moreover, the effect of both drugs on preformed biofilm of C. albicans strain was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay. The combination of the two antifungal compounds at 0·39 and 1 μg ml(-1) for bacillomycin D and amphotericin B, respectively, resulted in a clear enhancement of biofilm eradication compared to the results obtained with each drug alone. Furthermore, this combination was found to promote the closure of a gap produced in a monolayer of human keratinocytes. CONCLUSIONS Bacillomycin D and its combination with amphotericin B display impressive anti-biofilm and wound-healing activities. SIGNIFICANCE AND IMPACT OF THE STUDY Application of the lipopeptide bacillomycin D and the antifungal drug amphotericin B in medical devices may offer a promising alternative for topical treatment of Candida-associated infections in the setting of a wound. |
| Databáze: | OpenAIRE |
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