Downregulated LncRNA-ANCR promotes osteoblast differentiation by targeting EZH2 and regulating Runx2 expression
| Autor: | Pei-Cheng Xu, Lin Zhu |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Cellular differentiation
Biophysics Down-Regulation Core Binding Factor Alpha 1 Subunit Biology Biochemistry Cell Line Downregulation and upregulation medicine Transcriptional regulation Humans Enhancer of Zeste Homolog 2 Protein Promoter Regions Genetic Molecular Biology Regulation of gene expression Osteoblasts Cell growth EZH2 Polycomb Repressive Complex 2 Cell Differentiation Osteoblast Cell Biology RUNX2 Enhancer Elements Genetic medicine.anatomical_structure Gene Expression Regulation Cancer research RNA Long Noncoding |
| Zdroj: | Biochemical and Biophysical Research Communications. 432:612-617 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2013.02.036 |
| Popis: | Long noncoding RNAs (lncRNAs) are key regulators of diverse biological processes such as transcriptional regulation, cell growth and differentiation. Previous studies have demonstrated that the lncRNA-ANCR (anti-differentiation ncRNA) is required to maintain the undifferentiated cell state within the epidermis. However, little is known about whether ANCR regulates osteoblast differentiation. In this study, we found that the ANCR expression level is significantly decreased during hFOB1.19 cell differentiation. ANCR-siRNA blocks the expression of endogenous ANCR, resulting in osteoblast differentiation, whereas ANCR overexpression is sufficient to inhibit osteoblast differentiation. We further demonstrated that ANCR is associated with enhancer of zeste homolog 2 (EZH2) and that this association results in the inhibition of both Runx2 expression and subsequent osteoblast differentiation. These data suggest that ANCR is an essential mediator of osteoblast differentiation, thus offering a new target for the development of therapeutic agents to treat bone diseases. |
| Databáze: | OpenAIRE |
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