Overexpression of Egr2 and Egr4 protects rat brains against ischemic stroke by downregulating JNK signaling pathway
| Autor: | Xiao-Ping Shang, Rui-Na Niu, Jun-Fang Teng |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty MAP Kinase Signaling System Biochemistry 03 medical and health sciences symbols.namesake 0302 clinical medicine Western blot Downregulation and upregulation Internal medicine Medicine Animals Humans cardiovascular diseases Early Growth Response Protein 2 Inflammation Neurons medicine.diagnostic_test business.industry Kinase JNK Mitogen-Activated Protein Kinases Interleukin Infarction Middle Cerebral Artery General Medicine Transfection Rats Stroke Disease Models Animal 030104 developmental biology Endocrinology medicine.anatomical_structure nervous system Gene Expression Regulation cardiovascular system Nissl body symbols Cytokines Tumor necrosis factor alpha Neuron business 030217 neurology & neurosurgery |
| Zdroj: | Biochimie. 149 |
| ISSN: | 1638-6183 |
| Popis: | Objective The purpose of this study was to investigate the effect of Egr2 and Egr4 upregulation on ischemic stroke recovery of rats. Methods In this study, Sprague Dawley (SD) rats assigned at random into control, sham and MCAO (middle cerebral artery occlusion) group were treated accordingly to build MCAO models. The neurological severity scores (NSS) test was applied to assess rats' behavior. Triphenyltetrazolium chloride (TTC) staining reflected infarct areas while Nissl staining revealed the number of neurons. Levels of pro-inflammatory cytokines (interleukin [IL]-1β, IL-6 and tumor necrosis factor [TNF]-α) were judged by enzyme-linked immunosorbent assay (ELISA) in brain and serum tissues. We applied western blot to check the expression of Egr2, Egr4 and JNK/c-JUN (c-Jun N-terminal kinase) pathway. Further grouping of rats were based on various transfection, requiring control, sham, MCAO, MCAO + Egr2 cDNA (complementary DNA), MCAO + Egr4 cDNA, MCAO + Egr2 cDNA + Egr4 cDNA group to observe difference in MCAO recovery and JNK/c-JUN-pathway-related protein expression. Results Under successful modeling of MCAO, western blot results suggested down-regulation of Egr2 and Egr4 and overexpression of pro-inflammatory cytokines. The JNK/c-JUN pathway was activated. On upregulation of Egr2 and Egr4 in infarct areas, neurological function of SD rats recovered along with repressed JNK/c-JUN pathway activation and increased neuron number. Conclusion Upregulation of Egr2 and Egr4 could demote the activation of JNK/c-JUN pathway and the expression of pro-inflammatory cytokines in MCAO rats, so that Egr2 and Egr4 might be potential targets for ischemic stroke in future. |
| Databáze: | OpenAIRE |
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