Role of dynorphin and GABA in the inhibitory regulation of NMDA-induced dopamine release in striosome- and matrix-enriched areas of the rat striatum
| Autor: | M.L. Kemel, C. Gauchy, Jacques Glowinski, Marie-Odile Krebs, M. Desban |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Male
medicine.medical_specialty N-Methylaspartate Pyrrolidines Striosome Dopamine Population Receptors Opioid mu Tetrodotoxin (+)-Naloxone Dynorphin Bicuculline Tritium Dynorphins Rats Sprague-Dawley Internal medicine medicine Animals education gamma-Aminobutyric Acid Neurons Analgesics education.field_of_study Naloxone Chemistry Receptors Opioid kappa General Neuroscience 3 4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide (trans)-Isomer Articles Corpus Striatum Rats Kinetics Endocrinology nervous system NMDA receptor GABAergic medicine.drug |
| Zdroj: | Europe PubMed Central |
| ISSN: | 1529-2401 0270-6474 |
| DOI: | 10.1523/jneurosci.14-04-02435.1994 |
| Popis: | Using a new superfusion procedure in vitro, we have previously reported that the NMDA-evoked release of newly synthesized 3H-dopamine (DA) was higher in matrix- than in striosome-enriched areas of the rat striatum. In addition, GABAergic medium-sized spiny neurons were shown to be indirectly involved in this regulation. Since dynorphin and GABA are colocalized in a population of medium-sized spiny neurons, the role of dynorphin-containing neurons in the NMDA-evoked release of 3H-DA has been investigated using the same superfusion procedure on rat striatal slices. (1) The NMDA (50 microM, 25 min application)-evoked release of 3H-DA was increased in the presence of naloxone (1 microM, continuously delivered) in both striatal compartments, the overall response being more elevated in the striosome-enriched area. (2) The TTX (1 microM, continuously delivered)-resistant NMDA-evoked responses were also enhanced in the presence of naloxone, but in this case, the disinhibitory effects of naloxone were similar in striosome- and matrix- enriched areas. (3) The selective kappa-agonist U-50488 (1 microM) totally reversed the naloxone-disinhibitory effect on the NMDA-evoked response in the matrix-enriched area, but only partially in the striosome-enriched area. It also completely prevented the disinhibitory effect of naloxone on the TTX-resistant NMDA-evoked release of 3H-DA in both compartments. (4) The bicuculline (5 microM)- and naloxone (1 microM)-disinhibitory effects on the NMDA-evoked release of 3H-DA were additive in the matrix- but not in the striosome-enriched areas.(ABSTRACT TRUNCATED AT 250 WORDS) |
| Databáze: | OpenAIRE |
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