Arabidopsis glucosinolate storage cells transform into phloem fibres at late stages of development
Autor: | Barbara Ann Halkier, Pascal Hunziker, Alexander Schulz |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
0106 biological sciences
0301 basic medicine Physiology Glucosinolates S-cell Arabidopsis Plant Science Vacuole Plasmodesma Phloem 01 natural sciences 03 medical and health sciences symbols.namesake Arabidopsis thaliana phloem fibre biology Chemistry plasmodesmata Arabidopsis Proteins Endoplasmic reticulum food and beverages Golgi apparatus biology.organism_classification Research Papers Cell biology 030104 developmental biology photoactivation symbols phloem cap Secondary cell wall 010606 plant biology & botany |
Zdroj: | Journal of Experimental Botany Hunziker, P, Halkier, B A & Schulz, A 2019, ' Arabidopsis glucosinolate storage cells transform into phloem fibres at late stages of development ', Journal of Experimental Botany, vol. 70, no. 16, pp. 4305–4317 . https://doi.org/10.1093/jxb/erz176 |
ISSN: | 1460-2431 0022-0957 |
Popis: | Glucosinolate-storing S-cells are symplasmically coupled to glucosinolate-synthesizing cells and lignify during the final stages of differentiation, as revealed by a combination of improved TEM fixation and bioimaging. The phloem cap of Arabidopsis thaliana accumulates glucosinolates that yield toxic catabolites upon damage-induced hydrolysis. These defence compounds are stored in high concentrations in millimetre long S-cells. At early stages of development, S-cells initiate a process indicative of programmed cell death. How these cells are maintained in a highly turgescent state following this process is currently unknown. Here, we show that S-cells undergo substantial morphological changes during early differentiation. Vacuolar collapse and rapid clearance of the cytoplasm did not occur until senescence. Instead, smooth endoplasmic reticulum, Golgi bodies, vacuoles, and undifferentiated plastids were observed. Lack of chloroplasts indicates that S-cells depend on metabolite supply from neighbouring cells. Interestingly, TEM revealed numerous plasmodesmata between S-cells and neighbouring cells. Photoactivation of a symplasmic tracer showed coupling with neighbouring cells that are involved in glucosinolate synthesis. Hence, symplasmic transport might contribute to glucosinolate storage in S-cells. To investigate the fate of S-cells, we traced them in flower stalks from the earliest detectable stages to senescence. At late stages, S-cells were shown to deposit thick secondary cell walls and transform into phloem fibres. Thus, phloem fibres in the herbaceous plant Arabidopsis pass a pronounced phase of chemical defence during early stages of development. |
Databáze: | OpenAIRE |
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