Release behavior and intra-articular biocompatibility of celecoxib-loaded acetyl-capped PCLA-PEG-PCLA thermogels

Autor: Petit, Audrey, Sandker, Marjan, Müller, Benno, Meyboom, Ronald, van Midwoud, Paul, Bruin, Peter, Redout, Everaldo M, Versluijs-Helder, Marjan, van der Lest, Chris H A, Buwalda, Sytze J, de Leede, Leo G J, Vermonden, Tina, Kok, Robbert Jan, Weinans, Harrie, Hennink, Wim E, ES TR, Tissue Repair, Pharmaceutics, Sub General Pharmaceutics, Sub Immunopharmacology, LS Equine Muscoskeletal Biology, Sub Inorganic Chemistry and Catalysis, Sub Drug delivery
Přispěvatelé: Orthopedics and Sports Medicine, Child and Adolescent Psychiatry / Psychology, ES TR, Tissue Repair, Pharmaceutics, Sub General Pharmaceutics, Sub Immunopharmacology, LS Equine Muscoskeletal Biology, Sub Inorganic Chemistry and Catalysis, Sub Drug delivery
Rok vydání: 2014
Předmět:
Zdroj: Biomaterials, 35(27), 7919-7928. Elsevier
Biomaterials, 35(27), 7919. Elsevier Ltd
ISSN: 1878-5905
0142-9612
DOI: 10.1016/j.biomaterials.2014.05.064
Popis: In this study, we investigated the in vitro and in vivo properties and performance of a celecoxib-loaded hydrogel based on a fully acetyl-capped PCLA-PEG-PCLA triblock copolymer. Blends of different compositions of celocoxib, a drug used for pain management in osteoarthritis, and the acetyl-capped PCLA-PEG-PCLA triblock copolymer were mixed with buffer to yield temperature-responsive gelling systems. These systems containing up to 50 mg celecoxib/g gel, were sols at room temperature and converted into immobile gels at 37 degrees C. In vitro, release of celecoxib started after a similar to 10-day lag phase followed by a sustained release of similar to 90 days. The release was proven to be mediated by polymer dissolution from the gels. In vivo (subcutaneous injection in rats) experiments showed an initial celecoxib release of similar to 30% during the first 3 days followed by a sustained release of celecoxib for 4-8 weeks. The absence of a lag phase and the faster release seen in vivo were likely due to the enhanced celecoxib solubility in biological fluids and active degradation of the gel by macrophages. Finally, intra-articular biocompatibility of the 50 mg/g celecoxib-loaded gel was demonstrated using mu CT-scanning and histology, where no cartilage or bone changes were observed following injection into the knee joints of healthy rats. In conclusion, this study shows that celecoxib-loaded acetyl-capped PCLA-PEG-PCLA hydrogels form a safe drug delivery platform for sustained intra-articular release. (C) 2014 Elsevier Ltd. All rights reserved.
Databáze: OpenAIRE
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