The lipopolysaccharide model for the experimental induction of transient lameness and synovitis in Standardbred horses
| Autor: | Van de Water, E, Oosterlinck, M, Korthagen, N M, Duchateau, L, Dumoulin, M, van Weeren, P R, Olijve, J, van Doorn, D A, Pille, F, Equine Musculoskeletal Biology, dES RMSC, Dep Clinical Sciences, dES AVR, FAH voeding, dFAH AVR |
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| Přispěvatelé: | Equine Musculoskeletal Biology, dES RMSC, Dep Clinical Sciences, dES AVR, FAH voeding, dFAH AVR |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Lipopolysaccharides
040301 veterinary sciences Lameness Lameness Animal Arthritis Lipopolysaccharide Meloxicam Dinoprostone Injections Intra-Articular 0403 veterinary science Synovitis Joint disease Synovial Fluid Escherichia coli medicine Animals Synovial fluid Horses Veterinary Sciences Carpal Joint Carpal Joints General Veterinary business.industry Anti-Inflammatory Agents Non-Steroidal 0402 animal and dairy science Horse Repeated measures design 04 agricultural and veterinary sciences medicine.disease 040201 dairy & animal science veterinary(all) Disease Models Animal Anesthesia Horse Diseases Animal Science and Zoology business medicine.drug |
| Zdroj: | VETERINARY JOURNAL Veterinary Journal, 270. Bailliere Tindall Ltd |
| ISSN: | 1090-0233 1532-2971 |
| DOI: | 10.1016/j.tvjl.2021.105626 |
| Popis: | An established lipopolysaccharide (LPS) model previously described in Warmbloods, was inconsistent in Standardbred horses, where lameness was not detected despite the presence of synovitis. The present study aimed to determine the dose of LPS from E. coli O55:B5 required to induce mild to moderate lameness following middle carpal joint injection in Standardbred horses and to quantitate the induced lameness over time, with and without anti-inflammatory pre-treatment. In a baseline trial, eight healthy, clinically sound Standardbred horses were used in a rule-based dose-escalation design trial, starting at a dose of 10 endotoxin units (EU). Lameness at trot was evaluated visually and quantitatively (using an inertial-sensor system and pressure plate analysis). Synovial fluid aspirates were analysed for total nucleated cell counts, total protein and prostaglandin E2 (PGE2). Following 2 months wash-out, the effective LPS-dose determined in the baseline trial was used to evaluate the effect of anti-inflammatory treatment. A mixed model for repeated measures with horse as random effect was used for analysis. After injection of 10 EU LPS, the desired degree of lameness was observed in the baseline trial, with maximal lameness at post-injection hour (PIH) 4, followed by a rapid decline and return to baseline by PIH 48. No lameness was observed following pre-treatment with meloxicam. In synovial fluid, PGE2 was significantly higher at PIH 8 and PIH 24 in the baseline trial compared with following meloxicam pre-treatment. In conclusion, injection of the middle carpal joint with 10 EU LPS consistently induces a transient lameness and synovitis in Standardbred horses. |
| Databáze: | OpenAIRE |
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