Estimations of Rates of Synthesis and Degradation of Several Rat Mammary Gland Enzymes from Changes in Enzyme Activities
| Autor: | G. O. Korsrud, R. L. Baldwin |
|---|---|
| Rok vydání: | 1972 |
| Předmět: |
medicine.medical_specialty
Time Factors Hydrocortisone Uracil Nucleotides Malic enzyme Lyases Lactose Dehydrogenase Glucosephosphate Dehydrogenase Biology Citrate cleavage enzyme Mammary Glands Animal Malate Dehydrogenase Pregnancy Hexokinase Internal medicine Lactation Adrenal Glands medicine Animals Castration Citrates Isomerases UDPglucose pyrophosphorylase chemistry.chemical_classification Nucleoside Diphosphate Sugars Phosphogluconate Dehydrogenase Glucosephosphates Fatty acid Adrenalectomy General Medicine Rat Mammary Gland Nucleotidyltransferases Rats Glucose medicine.anatomical_structure Enzyme Endocrinology Hexosyltransferases chemistry Biochemistry Female Fatty Acid Synthases Half-Life |
| Zdroj: | Canadian Journal of Biochemistry. 50:386-391 |
| ISSN: | 0008-4018 |
| DOI: | 10.1139/o72-051 |
| Popis: | Based upon rates of decrease in the activities of citrate cleavage enzyme (EC 4.1.3.7), malic enzyme (EC 1.1.1.40), fatty acid synthetase, UDPglucose pyrophosphorylase (EC 2.7.7.9), UDPglucose 4-epimerase (EC 5.1.3.3), and glucose-6-phosphate dehydrogenase (EC 1.1.1.49) in rat mammary glands after adrenalectomy–ovariectomy on the 11th day of lactation, the half-lives of the enzymes were estimated to be 28, 31, 28, 50, 20, and 24 h, respectively. The half-life estimates for UDPglucose pyrophosphorylase and glucose-6-phosphate dehydrogenase compared favorably with previous estimates of 35 and 20 h, respectively, obtained in rats 5 days postpartum utilizing specific immunological techniques. In a second experiment, increases in the activities of enzymes in adrenalectomized, lactating rats after initiation of cortisol therapy were investigated. Rats were adrenalectomized on the 5th day of lactation and cortisol therapy was started 5 days later. The estimated half-lives for citrate cleavage enzyme, malic enzyme, fatty acid synthetase, UDPglucose pyrophosphorylase, UDPglucose 4-epimerase, the A protein of the lactose synthetase complex, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase (EC 1.1.1.44), and hexokinase (EC 2.7.1.1) were, respectively, 84, 60, 92, 76, 170, 102, 79, 88, and 81 h. |
| Databáze: | OpenAIRE |
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