Conductive Composite Biosensor System for Electrochemical Indinavir Drug Detection
Autor: | Nicolette Hendricks-Leukes, Rachel Fanelwa Ajayi, Emmanuel I. Iwuoha, Priscilla G. L. Baker, Natasha Ross |
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Jazyk: | angličtina |
Rok vydání: | 2015 |
Předmět: |
Drug
medicine.diagnostic_test Combination therapy Article Subject Chemistry media_common.quotation_subject General Chemistry Pharmacology Polypyrrole Combinatorial chemistry lcsh:Chemistry Drug detection chemistry.chemical_compound lcsh:QD1-999 Therapeutic drug monitoring Indinavir medicine Protease inhibitor (pharmacology) Biosensor media_common medicine.drug |
Zdroj: | Journal of Chemistry, Vol 2015 (2015) |
ISSN: | 2090-9063 |
DOI: | 10.1155/2015/630408 |
Popis: | Indinavir is a protease inhibitor antiretroviral (ARV) drug, which forms part of the highly active antiretroviral therapy during the treatment of HIV/AIDS. Indinavir undergoes first-pass metabolism through the cytochrome P450 (CYP) enzymes in the human liver, of which CYP3A4 is the most influential isoenzyme. Multidrug combination therapy and, as such, therapeutic drug monitoring (TDM) during HIV/AIDS treatment are therefore critical, to prevent adverse interactions. The conventional sensitive and specific assays available for quantifying ARV drugs, however, suffer from distinct disadvantages. In this regard, biosensors can be used to provide real time information on the metabolic profile of the drug. In this study, a biosensor with cobalt(III) sepulchrate trichloride{CoSep3+}as diffusional mediator was constructed. The biosensor platform consisted of CYP3A4 immobilized onto a gold nanoparticle (GNP) overoxidized polypyrrole (OvOxPpy) carrier matrix. The biosensor exhibited reversible electrochemistry, with formal potential determined as −624 ± 5 mV, from voltammetric analysis, with overall electron transfer being diffusion controlled. The biosensor showed typical electrocatalytic response to dioxygen (O2), exemplified by the distinct increase in the cathodic peak current (Ip,c). A concentration-dependent increase inIp,cwas observed in response to consecutive additions of Indinavir. |
Databáze: | OpenAIRE |
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