The Persistence of Electrostatically Seeded Endothelial Cells Lining a Small Diameter Expanded Polytetrafluoroethylene Vascular Graft
| Autor: | Raymond G. Makhoul, Charles Fields, Gary L. Bowlin, Cynthia Allen, Anthony D. Cassano, Stanley E. Rittgers, Andrew D. Meyer |
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| Rok vydání: | 2001 |
| Předmět: |
Materials science
Small diameter Static Electricity 0206 medical engineering Biomedical Engineering 02 engineering and technology Expanded polytetrafluoroethylene 021001 nanoscience & nanotechnology 020601 biomedical engineering Blood Vessel Prosthesis Biomaterials Endothelial stem cell Dogs Microscopy Electron Scanning Animals Female Endothelium Vascular 0210 nano-technology Polytetrafluoroethylene Vascular graft Biomedical engineering |
| Zdroj: | Journal of Biomaterials Applications. 16:157-173 |
| ISSN: | 1530-8022 0885-3282 |
| Popis: | Purpose: The purpose of this study was to evaluate the persistence of electrostatically seeded endothelial cells (ECs) lining an expanded polytetrafluorethylene (e-PTFE) graft after one week exposure to in vivo circulation in a canine femoral artery bypass model. This was accomplished by visualizing the PKH 26 (red fluorescent) label placed in the EC membranes prior to the seeding procedure. Furthermore, this study was performed to confirm that thesourceof the ECs lining thegraft werethosefrom theinitial inoculum. Methods: This evaluation consisted of harvesting autologous, canine jugular vein ECs, PKH 26 labeling of the ECs, electrostatic EC seeding the e-PTFE grafts (4 mm GORE-TEX®, Length = 6 cm), implanting thegrafts (femoral artery model) for one week, and explanting the grafts for light, fluorescent and scanning electron microscopy evaluations of the luminal surface. Results: The unseeded grafts (controls) had a mean fluorescence surface coverage of 6.82 ± 7.19%, while the EC seeded grafts had a mean of 90.3 ± 14.3% which is significantly (p Conclusions: The electrostatically seeded ECs persisted after implantation of the graft as demonstrated by the PKH 26 labeling data. The fluorescent data also demonstrated that the neointima formed (EC luminal surface coverage) one week after implantation was in fact derived from the ECs initially seeded as determined by the abundance of the labeled ECs. |
| Databáze: | OpenAIRE |
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