Synergistic activation of astrocytes by ATP and norepinephrine in the rat supraoptic nucleus

Autor: O. Solovieva, J. Espallergues, Nicolas Hussy, K. Bauer, Anne Vincent, V. Técher, Gérard Alonso
Přispěvatelé: Mécanismes moléculaires dans les démences neurodégénératives (MMDN), Université de Montpellier (UM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Santé et de la Recherche Médicale (INSERM)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL), Laboratoire de Chimie et Biologie des Métaux (LCBM - UMR 5249), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Max-Planck-Institut, Institut de Génomique Fonctionnelle (IGF), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), Université Montpellier 2 - Sciences et Techniques (UM2)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2007
Předmět:
Male
Oxytocin
Supraoptic nucleus
hypothalamo-neurohypophysial system
Norepinephrine
Receptors
Purinergic P2Y1
chemistry.chemical_compound
Adenosine Triphosphate
0302 clinical medicine
hypothalamus
Receptor
Neurotransmitter
Chelating Agents
Neurons
0303 health sciences
Neurosecretion
General Neuroscience
[SDV.BA]Life Sciences [q-bio]/Animal biology
Purinergic receptor
Receptors
Adrenergic
medicine.anatomical_structure
Neuroglia
[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]
Supraoptic Nucleus
Sodium Channel Blockers
medicine.drug
Astrocyte
Hypothalamo-Hypophyseal System
medicine.medical_specialty
Adrenergic receptor
Vasopressins
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
Biology
03 medical and health sciences
Internal medicine
purinergic receptors
medicine
Animals
Calcium Signaling
Rats
Wistar
030304 developmental biology
Receptors
Purinergic P2
calcium transients
Rats
Endocrinology
chemistry
Astrocytes
Calcium
adrenergic receptors
030217 neurology & neurosurgery
Zdroj: Neuroscience
Neuroscience, Elsevier-International Brain Research Organization, 2007, 148 (3), pp.712-723. ⟨10.1016/j.neuroscience.2007.03.043⟩
Neuroscience, 2007, 148 (3), pp.712-723. ⟨10.1016/j.neuroscience.2007.03.043⟩
ISSN: 0306-4522
1873-7544
DOI: 10.1016/j.neuroscience.2007.03.043⟩
Popis: International audience; Supraoptic nucleus (SON) neurons receive a dense innervation from noradrenergic fibers, the activity of which stimulates vasopressin (VP) and oxytocin (OT) release, notably during homeostatic regulation of blood pressure and volume. This regulation is known to involve the co-release of norepinephrine (NE) and ATP, which act in synergy to stimulate Ca2+ increase in SON neurons and to enhance release of VP and OT from hypothalamo-neurohypophysial explants. We here demonstrate that both ATP and NE also trigger transient intracellular Ca2+ rise in rat SON astrocytes, the two agonists showing a synergistic action similarly to what has been reported in SON neurons. The responses to both agonists are not or are only moderately affected after blockade of neuronal activity by tetrodotoxin, or of neurotransmitter release by removal of extracellular Ca2+, suggesting that the receptors involved are located on the astrocytes themselves. ATP acts via P2Y1 receptors, as indicated by the pharmacological profile of Ca2+ responses and the strong immunolabeling for this receptor in SON astrocytes. Responses to NE involve both α and β adrenergic receptors, the latter showing a permissive role on the former. These results point to further implication of SON astrocytes in the regulation of VP and OT secretion, and suggest that they are potentially important elements participating in all regulatory processes of hypothalamo-neurohypophysial function that involve activation of noradrenergic pathways.
Databáze: OpenAIRE
Externí odkaz: