Glucosylceramide Synthase Activity and Ceramide Levels are Modulated during Cerebral Ischemia after Ischemic Preconditioning
| Autor: | Yong Chen, Irene Ginis, Kenzo Takahashi, R. F. White, Ryoji Nishioka, Frank C. Barone, John M. Hallenbeck, Dace Klimanis |
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| Rok vydání: | 2004 |
| Předmět: |
Male
medicine.medical_specialty Ceramide Ischemia Glucosylceramide synthase Ceramides Brain Ischemia 030218 nuclear medicine & medical imaging Central nervous system disease 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Rats Inbred SHR Internal medicine medicine Animals Middle cerebral artery occlusion Ischemic Preconditioning ATP synthase biology business.industry Brain Infarction Middle Cerebral Artery medicine.disease Rats Endocrinology Neurology chemistry Glucosyltransferases Anesthesia biology.protein Ischemic preconditioning Neurology (clinical) Cardiology and Cardiovascular Medicine business 030217 neurology & neurosurgery Glucosylceramide synthase activity |
| Zdroj: | Journal of Cerebral Blood Flow & Metabolism. 24:623-627 |
| ISSN: | 1559-7016 0271-678X |
| Popis: | After 24-hour middle cerebral artery occlusion (MCAO) in spontaneously hypertensive rats, brain ceramide level increased from baseline reached 595% (ischemic core) and 460% (perifocal/penumbral areas); brain glucosylceramide synthase (GCS) activities in these areas simultaneously decreased by 70% and 50%, respectively. Ten-minute MCAO preconditioning significantly attenuated 24-hour MCAO-induced ceramide accumulation by 40% to 60% in ischemic core and perifocal areas, and GCS activities improved by 60% to 70% in both areas. Thus, potentially toxic levels of brain ceramide induced by MCAO were attenuated to intermediate levels in preconditioned animals; brain GCS activity was relatively preserved. In ischemic tolerance, GCS appears to modulate otherwise high levels of brain ceramide. |
| Databáze: | OpenAIRE |
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