Application of oxygen-independent biosensor for testing yeast fermentation capacity
| Autor: | Irina Bachmatova, Liucija Marcinkeviciene, Bogumila Kurtinaitiene, Julija Razumiene, Vidute Gureviciene, Vytautas Melvydas, Rolandas Meškys, Valdas Laurinavicius |
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| Rok vydání: | 2010 |
| Předmět: |
Conductometry
PQQ Cofactor Biomedical Engineering Biophysics Biosensing Techniques Saccharomyces cerevisiae chemistry.chemical_compound Oxygen Consumption Pyrroloquinoline quinone Glucose dehydrogenase Electrochemistry Electrodes Biological Oxygen Demand Analysis Detection limit Chemistry Glucose 1-Dehydrogenase Equipment Design General Medicine Yeast Equipment Failure Analysis Yeast in winemaking Glucose Linear range Biochemistry Fermentation Biosensor Biotechnology |
| Zdroj: | Biosensors and Bioelectronics. 26:766-771 |
| ISSN: | 0956-5663 |
| DOI: | 10.1016/j.bios.2010.06.038 |
| Popis: | The pyrroloquinoline quinone (PQQ)-dependent soluble glucose dehydrogenase based carbon paste electrodes were investigated and applied for glucose monitoring in the oxygen deficient media. Reagentless biosensors possessing a wide linear range (up to 5 mM glucose with a detection limit of 0.12 mM) were designed. The oxygen-insensitive response of the biosensor creates the opportunity to use it as a flow-through device for continuous monitoring of glucose in media during the wine yeast fermentation process. The analysis of glucose assimilation rate by yeast strains using the developed biosensor correlated well (R2=0.9938) with convenient yeast testing methods. |
| Databáze: | OpenAIRE |
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