Elastic modulus and migration capability of drug treated leukemia cells K562
| Autor: | Kui Wang, Yuanjun Zhang, Bin Tang, Yuntian Xue, Ye Tian, Alfonso H.W. Ngan, Xiangchao Pang, L.I. Ruiz-Ortega, Ying Peng |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Biophysics Antineoplastic Agents Tretinoin Vimentin Biochemistry Dexamethasone 03 medical and health sciences 0302 clinical medicine Cell Movement Elastic Modulus hemic and lymphatic diseases medicine Humans Cyclophosphamide Molecular Biology Elastic modulus Leukemia biology Chemistry Leukostasis Cell Biology medicine.disease Biomechanical Phenomena Haematopoiesis 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis biology.protein Cancer research Tetradecanoylphorbol Acetate Bone marrow Stem cell K562 Cells K562 cells |
| Zdroj: | Biochemical and Biophysical Research Communications. 516:177-182 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2019.06.024 |
| Popis: | Leukemia is a commonly seen disease caused by abnormal differentiation of hematopoietic stem cells and blasting in bone marrow. Despite drugs are used to treat the disease clinically, the influence of these drugs on leukemia cells’ biomechanical properties, which are closely related to complications like leukostasis or infiltration, is still unclear. Due to non-adherent and viscoelastic nature of leukemia cells, accurate measurement of their elastic modulus is still a challenging issue. In this study, we adopted rate-jump method together with optical tweezers indentation to accurately measure elastic modulus of leukemia cells K562 after phorbol 12-myristate 13-acetate (PMA), all-trans retinoic acid (ATRA), Cytoxan (CTX), and Dexamethasone (DEX) treatment, respectively. We found that compared to control sample, K562 cells treated by PMA showed nearly a threefold increase in elastic modulus. Transwell experiment results suggested that the K562 cells treated with PMA have the lowest migration capability. Besides, it was shown that the cytoskeleton protein gene α-tubulin and vimentin have a significant increase in expression after PMA treatment by qPCR. The results indicate that PMA has a significant influence on protein expression, stiffness, and migration ability of the leukemia cell K562, and may also play an important role in the leukostasis in leukemia. |
| Databáze: | OpenAIRE |
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