Effect of freezing bull semen in two non-egg yolk extenders on post-thaw sperm quality
| Autor: | Anders Johannisson, Anders Edman, Jane M. Morrell, F Dórea, Isabel B. Lima-Verde, Ziyad Al-Kass, Thanapol Nongbua, Theodoros Ntallaris, Essraa M Al-Essawe, K Kupisiewicz, Nils Lundeheim |
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| Rok vydání: | 2017 |
| Předmět: |
Male
food.ingredient medicine.medical_treatment Semen Cryopreservation law.invention Andrology 03 medical and health sciences fluids and secretions 0302 clinical medicine Endocrinology food Cryoprotective Agents law Yolk Lecithins medicine Image Processing Computer-Assisted Animals Insemination Artificial chemistry.chemical_classification Membrane Potential Mitochondrial Reactive oxygen species Liposome 030219 obstetrics & reproductive medicine urogenital system Chemistry Artificial insemination Extender Cell Membrane 0402 animal and dairy science Vitamin K 3 04 agricultural and veterinary sciences 040201 dairy & animal science Sperm Spermatozoa Semen Analysis Liposomes Animal Science and Zoology Cattle Female Soybeans Biotechnology Semen Preservation |
| Zdroj: | Reproduction in domestic animals = Zuchthygiene. 53(1) |
| ISSN: | 1439-0531 |
| Popis: | Contents Traditionally, extenders for bull semen included egg yolk or milk, but recently there has been a move to avoid material of animal origin. The aim of this study was to evaluate the effects of two commercial extenders (based on soya lecithin and liposomes) on bull sperm quality after cryopreservation. Post-thaw sperm quality was evaluated by computer-assisted sperm analysis and flow cytometric assessment of membrane integrity, chromatin integrity, mitochondrial membrane potential, production of reactive oxygen species and tyrosine phosphorylation. Furthermore, an artificial insemination (AI) trial was conducted, and 56-day non-return rates were evaluated. Semen frozen in the liposome-based extender showed similar membrane integrity and higher mitochondrial membrane potential compared to those in the soya lecithin-based extender. Chromatin integrity and production of live H2O2+ reactive oxygen species were similar in both extenders. Less superoxide was produced in the samples extended with liposome-based extender, with or without menadione stimulation. Chromatin integrity and tyrosine phosphorylation were not affected by either type of extender. No differences in 56-day non-return rate between extenders containing soya lecithin and liposomes were observed in the AI trial (66% ± 0.8 and 65% ± 0.8, respectively). In conclusion, the sperm quality of bull semen frozen in the two extenders that do not contain material of animal origin was similar, although the semen frozen in the liposome-based extender had higher mitochondrial membrane potential. Either extender could be used in situations where extenders containing material of animal origin are to be avoided. |
| Databáze: | OpenAIRE |
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